Gastric Cancer
Copyright ©The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 15, 2003; 9(9): 1904-1908
Published online Sep 15, 2003. doi: 10.3748/wjg.v9.i9.1904
Inhibitory effect of octreotide on gastric cancer growth via MAPK pathway
Chun-Hui Wang, Cheng-Wei Tang, Chun-Lun Liu, Li-Ping Tang
Chun-Hui Wang, Cheng-Wei Tang, Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China
Chun-Lun Liu, Li-Ping Tang, Department of Gastroenterology, The First Hospital, Chongqing University of Medical Sciences, Chongqing 400016, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China for Excellent Young Scientists, No. 39725012 and the National Natural Science Foundation of China, No. 30170418
Correspondence to: Cheng-Wei Tang, Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China. cwtang@medmail.com.cn
Telephone: +86-28-85422383
Received: April 7, 2003
Revised: April 23, 2003
Accepted: May 19, 2003
Published online: September 15, 2003
Abstract

AIM: Somatostatin and its analogues may suppress the growth of various tumor cells. However, the effect of octreotide on growth of gastric adenocarcinoma is still largely unknown. This study was to explore if octreotide could inhibit the growth of gastric adenocarcinoma and its probable mechanisms.

METHODS: Proliferation of gastric cancer cell line affected by octreotide was determined by 3H-thymidine incorporation. After xenografts of human gastric cancer were implanted orthotopically in stomach, nude mice were administrated octreotide for 8 weeks. The mRNA of somatostatin receptor in the SGC-7901 cells was detected by reverse transcription polymerase chain reaction technique. Extracellular signal-regulated protein kinase and c-Fos in gastric cancer tissues were measured by immunohistochemistry and Western blot. Activator protein-1 binding activity was examined by electrophoretic mobility sift assay.

RESULTS: 3H-thymidine incorporation into SGC-7901 cells was significantly decreased by octreotide in a concentration dependent manner. Either size or weight of tumors treated with octreotide was significantly reduced in vivo. The inhibition rate for tumor was 62.3% in octreotide group. The genes of somatostatin receptors 2 and 3 were expressed in SGC-7901 gastric cancer cell lines. Extracellular signal-regulated protein kinase and c-Fos protein level were decreased in gastric adenocarcinoma treated with octreotide. Moreover, fetal calf serum stimulated activator protein-1 binding activity could be suppressed by octreotide potentially.

CONCLUSION: Inhibition of sequential molecular events in MAPK pathway may interpret the mechanisms underlying the effect of octreotide on the growth of gastric adenocarcinoma.

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