Basic Research
Copyright ©The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Aug 15, 2003; 9(8): 1772-1776
Published online Aug 15, 2003. doi: 10.3748/wjg.v9.i8.1772
Role of TFF in healing of stress-induced gastric lesions
Shi-Nan Nie, Xiao-Ming Qian, Xue-Hao Wu, Shi-Yu Yang, Wen-Jie Tang, Bao-Hua Xu, Fang Huang, Xin Lin, Dong-Yan Sun, Hai-Chen Sun, Zhao-Shen Li
Shi-Nan Nie, Xiao-Ming Qian, Xue-Hao Wu, Shi-Yu Yang, Wen-Jie Tang, Bao-Hua Xu, Fang Huang, Xin Lin, Dong-Yan Sun, Hai-Chen Sun, Emergency Department, Nanjing General Hospital of Nanjing Command/Clinical School of Medical College of Nanjing University, Nanjing 210002, Jiangsu Province, China
Zhao-Shen Li, Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai, 200433, China
Author contributions: All authors contributed equally to the work.
Supported by the Key Project of the Tenth-Five-Year Plan Foundation of PLA, No.01Z059
Correspondence to: Shi-Nan Nie, Emergency Department, Nanjing General Hospital of Nanjing Command/Clinical School of Medical College of Nanjing University, Nanjing 210002, Jiangsu Province, China. shnnie630504@.sohu.com
Telephone: +86-25-4826808-58143
Received: March 5, 2003
Revised: March 22, 2003
Accepted: April 1, 2003
Published online: August 15, 2003
Abstract

AIM: To determine the changes of pS2 and ITF of TFF expression in gastric mucosa and the effect on ulcer healing of pS2, ITF to Water-immersion and restraint stress (WRS) in rats.

METHODS: Wistar rats were exposed to single or repeated WRS for 4 h every other day for up to 6 days.Gastric mucosal blood flow (GMBF) was measured by LDF-3 flowmeter and the extent of gastric mucosal lesions were evaluated grossly and histologically. Expression of pS2 and ITF mRNA was determined by RT-PCR. Immunohistochemistry was used to further detect the expression of pS2 and ITF.

RESULTS: WRS applied once produced numerous gastric mucosal erosions, but the number of these lesions gradually declined and GMBF restored at 2, 4, 8 h after stress. The area of gastric mucosal lesion was reduced by 64.9% and GMBF was increased by 89.8% at 8 h. The healing of stress-induced ulcerations was accompanied by increased expression of pS2 (0.51 ± 0.14 vs 0.77 ± 0.11, P < 0.01) and ITF (0.022 ± 0.001vs 0.177 ± 0.010, P < 0.01). The results were demonstrated further by immunohistochemistry of pS2 (0.95 ± 0.11 vs 1.41 ± 0.04, P < 0.01) and ITF (0.134 ± 0.001 vs 0.253 ± 0.01,P < 0.01). With repeated WRS, adaptation to this WRS developed, the area of gastric mucosal lesions was reduced by 22.0% after four consecutive WRS. This adaptation to WRS was accompanied by increased GMBF (being increased by 94.2%), active cell proliferation in the neck region of gastric glands, and increased expression of pS2 (0.37 ± 0.02 vs 0.77 ± 0.01, P < 0.01) and ITF (0.040 ± 0.001vs 0.372 ± 0.010, P < 0.01). The result was demonstrated further by immunohistochemistry of pS2 (0.55 ± 0.04 vs 2.46 ± 0.08, P < 0.01) and ITF (0.134 ± 0.001vs 0.354 ± 0.070, P < 0.01).

CONCLUSION: TFF may not only participate in the early phase of epithelial repair known as restitution(maked by increased cell migration),but also play an important role in the subsequent,protracted phase of glandular renewal(made by cell proliferation).

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