Brief Reports
Copyright ©The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jul 15, 2003; 9(7): 1607-1610
Published online Jul 15, 2003. doi: 10.3748/wjg.v9.i7.1607
Effect of P-selectin monoclonal antibody on metastasis of gastric cancer and immune function
Jin-Lian Chen, Wei-Xiong Chen, Jin-Shui Zhu, Ni-Wei Chen, Tong Zhou, Ming Yao, Dong-Qing Zhang, Yun-Lin Wu
Jin-Lian Chen, Wei-Xiong Chen, Jin-Shui Zhu, Ni-Wei Chen, Department of Gastroenterology, Shanghai Jiao-Tong University affiliated Sixth People’s Hospital, Shanghai 200233, China
Tong Zhou, Yun-Lin Wu, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China
Ming Yao, Shanghai Cancer Institute, Shanghai 200233, China
Dong-Qing Zhang, Shanghai Institute of Immunology, Shanghai 200025, China
Author contributions: All authors contributed equally to the work.
Supported by the Youth Science Foundation of Shanghai Public AdministrationNo.131984Y15
Correspondence to: Jin-Lian Chen, Department of Gastroenterology, Shanghai Sixth People’s Hospital, Shanghai Jiao-Tong University affiliated Sixth People’s Hospital, Shanghai 200233, China. wqq_021002@163.com
Telephone: +86-21-64369181
Received: March 2, 2003
Revised: March 14, 2003
Accepted: March 29, 2003
Published online: July 15, 2003
Abstract

AIM: To investigate the effect of cell adhesion molecule P-selectin monoclonal antibody (Mab) on metastasis and immune function of mice orthototopically implanted with human gastric cancer tissue.

METHODS: SCID mice were implanted orthotopically with SGC-7901 human gastric carcinoma tissue. Starting from day 3 after operation, animals were given intravenously PBS or P-selectin Mab (100 μg/injection) (for both normal mice and tumor-implanted mice with tumors), twice weekly for 3 wk. Two animals in each group were sacrificed randomly at the 1st, 2nd, 4th week and 6th week. While T cell and B cell transformation indices were determined with the 3H TdR infiltration method, the NK cell activity was detected by the LDH release method.

RESULTS: The metastatic rate in the P-selectin Mab treated group was lower than that in the PBS treated group (with tumors). The NK activity of normal mice increased over time. The immune functions (T, B cell function, NK activity) of the tumor group in the 6th week were significantly lower than those in the 4th week, but the change was attenuated by P-selectin Mab.

CONCLUSION: P-selectin Mab could suppress the metastasis of gastric cancer with no adverse effect on host immune function.

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