Zhang LW, Ren J, Zhang L, Zhang HM, Jin B, Pan BR, Si XM, Zhang YJ, Wang ZH, Pan YL, Festein SM. Construction and expression of recombined human AFP eukaryotic expression vector. World J Gastroenterol 2003; 9(7): 1465-1468 [PMID: 12854142 DOI: 10.3748/wjg.v9.i7.1465]
Corresponding Author of This Article
Dr. Jun Ren, Department of Oncology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China. renjun@fmmu.edu.cn
Article-Type of This Article
Liver Cancer
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World J Gastroenterol. Jul 15, 2003; 9(7): 1465-1468 Published online Jul 15, 2003. doi: 10.3748/wjg.v9.i7.1465
Construction and expression of recombined human AFP eukaryotic expression vector
Li-Wang Zhang, Jun Ren, Liang Zhang, Hong-Mei Zhang, Bin Jin, Bo-Rong Pan, Xiao-Ming Si, Yan-Jun Zhang, Zhong-Hua Wang, Yang-Lin Pan, Stephen M Festein
Li-Wang Zhang, Jun Ren, Liang Zhang, Hong-Mei Zhang, Bin Jin, Bo-Rong Pan, Xiao-Ming Si, Yan-Jun Zhang, Department of Oncology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
Zhong-Hua Wang, Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
Yang-Lin Pan, Department of Gastroenterology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
Stephen M Festein, Department of Biology, Hamiliton College, 198 College Hill Road Clinton, New York 13323, USA
Author contributions: All authors contributed equally to the work.
Supported by Teaching and Research Award Program for Outstanding Young Teacher in Higher Education Institutes, No.TRAPOYT99-016, and the Fund for Distinguished Young Scholars of Chinese PLA, No.01J016
Correspondence to: Dr. Jun Ren, Department of Oncology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China. renjun@fmmu.edu.cn
Telephone: +86-29-3375407
Received: January 11, 2003 Revised: March 1, 2003 Accepted: March 5, 2003 Published online: July 15, 2003
Abstract
AIM: To construct a recombined human AFP eukaryotic expression vector for the purpose of gene therapy and target therapy of hepatocellular carcinoma (HCC).
METHODS: The full length AFP-cDNA of prokaryotic vector was digested, and subcloned to the multi-clony sites of the eukaryotic vector. The constructed vector was confirmed by enzymes digestion and electrophoresis, and the product expressed was detected by electrochemiluminescence and immunofluorescence methods.
RESULTS: The full length AFP-cDNA successfully cloned to the eukaryotic vector through electrophoresis, 0.9723 IU/mL AFP antigen was detected in the supernatant of AFP-CHO by electrochemiluminescence method. Compared with the control groups, the differences were significant (P < 0.05). AFP antigen molecule was observed in the plasma of AFP-CHO by immunofluorescence staining.
CONCLUSION: The recombined human AFP eukaryotic expression vector can express in CHO cell line. It provides experimental data for gene therapy and target therapy of hepatocellular carcinoma.
