Gastric Cancer
Copyright ©The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jul 15, 2003; 9(7): 1431-1434
Published online Jul 15, 2003. doi: 10.3748/wjg.v9.i7.1431
Expression of NGF family and their receptors in gastric carcinoma: A cDNA microarray study
Jian-Jun Du, Ke-Feng Dou, Shu-You Peng, Bing-Zhi Qian, Hua-Sheng Xiao, Feng Liu, Wei-Zhong Wang, Wen-Xian Guan, Zhi-Qing Gao, Ying-Bin Liu, Ze-Guang Han
Jian-Jun Du, Ke-Feng Dou, Wei-Zhong Wang, Wen-Xian Guan, Zhi-Qing Gao, Department of General Surgery, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, Shanxi Province, China
Shu-You Peng, Ying-Bin Liu, Department of General Surgery, the Second Affiliated Hospital, Medical College, Zhejiang University, Hangzhou 310009, Zhejiang Province, China
Bing-Zhi Qian, Hua-Sheng Xiao, Feng Liu, Ze-Guang Han, China Human Genome Center at Shanghai, Shanghai 201203, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Ze-Guang Han, China Human Genome Center at Shanghai, 351 Guo Shou Jing Road, Zhangjiang High-Tech Park, Shanghai 201203, China. hanzg@chgc.sh.cn
Telephone: +86-29-3375256
Received: February 26, 2003
Revised: March 4, 2003
Accepted: March 28, 2003
Published online: July 15, 2003
Abstract

AIM: To investigate the expression of NGF family and their receptors in gastric carcinoma and normal gastric mucosa, and to elucidate their effects on gastric carcinoma.

METHODS: RNA of gastric cancer tissues and normal gastric tissues was respectively isolated and mRNA was purified. Probes of both mRNA reverse transcription product cDNAs labled with α-33P dATP were respectively hybridized with Atlas Array membrane where NGF and their family genes were spotted on. Hybridized signal images were scanned on phosphor screen with ImageQuant 5.1 software after hybridization. Normalized values on spots were analyzed with ArrayVersion 5.0 software. Differential expression of NGF family and their receptors mRNA was confirmed between hybridized Atlas Array membranes of gastric cancer tissues and normal gastric mucosa, then their effects on gastric carcinoma were investigated.

RESULTS: Hybridization signal images on Atlas Array membrane appeared in a lower level of nonspecific hybridization. Both of NGF family and their receptors Trk family mRNA were expressed in gastric cancer and normal gastric mucosa. But adversely up-regulated expression in other tissues and organs. NGF, BDGF, NT-3, NT-4/5, NT-6 and TrkA, B and C were down-regulated simultaneously in gastric carcinoma in comparison with normal gastric mucosa. Degrees of down-regulation in NGF family were greater than those in their receptors Trk family. Down-regulation of NT-3 and BDGF was the most significant, and TrkC down-regulation level was the lowest in receptors Trk family.

CONCLUSION: Down-regulated expression of NGF family and their receptors Trk family mRNA in gastric cancer is confirmed. NGF family and their receptors Trk family probably play a unique role in gastric cancer cell apoptosis by a novel Ras or Raf signal transduction pathway. Their synchronous effects are closely associated with occurrence and development of gastric carcinoma induced by reduction of signal transduction of programmed cell death.

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