Esophageal Cancer
Copyright ©The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jul 15, 2003; 9(7): 1394-1397
Published online Jul 15, 2003. doi: 10.3748/wjg.v9.i7.1394
CYP1A1, GSTs and mEH polymorphisms and susceptibility to esophageal carcinoma: Study of population from a high- incidence area in north China
Li-Dong Wang, Shu Zheng, Bin Liu, Jian-Xiang Zhou, Yan-Jie Li, Ji-Xue Li
Li-Dong Wang, Cancer Institute, Zhejiang University, Hangzhou, 310009, Zhejiang Province, China and Laboratory for Cancer Research, College of Medicine, Zhengzhou University, Zhengzhou, 450052, Henan Province, China
Shu Zheng, Cancer Institute, Zhejiang University, Hangzhou, 310009, Zhejiang Province, China
Jian-Xiang Zhou, Yan-Jie Li, Ji-Xue Li, Laboratory for Cancer Research, College of Medicine, Zhengzhou University, Zhengzhou, 450052, Henan Province, China
Bin Liu, Department of Gastroenterology, Tongren Hospital, Capital Medical University, Beijing 100013, China
Author contributions: All authors contributed equally to the work.
Supported by National Outstanding Young Scientist Award of China 30025016 (China), State Key Project for Basic Research G1998051206 (China), Foundation of Henan Education Committee 1999125 and the U.S. NIH Grant CA65871
Correspondence to: Li-Dong Wang, M.D., Professor of Pathology and Oncology, Invited Professor of Henan Province, Director of Laboratory for Cancer Research, College of Medicine, Zhengzhou University, Zhengzhou, 450052, Henan Province, China. lidong0823@sina.com
Telephone: +86-11-371-6970165 Fax: +86-11-371-6970165
Received: March 12, 2003
Revised: March 24, 2003
Accepted: May 11, 2003
Published online: July 15, 2003
Abstract

AIM: To characterize cytochrome P4501A1 (CYP1A1), glutathione S-transferases (GSTs) and microsomal epoxide hydrolase (mEH) polymorphisms in Chinese esophageal cancer patients.

METHODS: Multiplex polymerase chain reaction (PCR) and PCR based restriction fragment length polymorphisms (PCR-RFLP) were used to detect polymorphism changes of CYP, GSTs and mEH on esophageal cancerous and precancerous lesions as well as in case control group. All the examination samples were obtained from Linzhou (formerly Linxian), Henan Province, the highest incidence area for esophageal cancer.

RESULTS: The frequency of CYP1A1 3’ polymorphism in case control group (26/38, 68%) was significantly higher than in esophageal squamous cell carcinoma group (ESCC) (29/62, 47%) (P < 0.05). A significant difference in the incidence of mEH slow allele variant was observed between case control group (15/38, 39%) and esophageal dysplasia group (22/32, 69%) or ESCC group (39/62, 63%) (P < 0.05). However, no significant difference was observed among different groups in the polymorphisms of CYP1A1 exon 7, GSTM1, GSTT1, GSTP1 and mEH fast allele.

CONCLUSION: The present results suggest that CYP1A1 3’ polymorphism may be one of the promising protective factors and its wild gene type may be an indicator for higher susceptibility to esophageal cancer. mEH slow allele variant, associated with the progression of esophageal precancerous lesions, may contribute to the high susceptibility to esophageal carcinoma.

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