Gastric Cancer
Copyright ©The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. May 15, 2003; 9(5): 905-909
Published online May 15, 2003. doi: 10.3748/wjg.v9.i5.905
Relationship between inactivation of p16 gene and gastric carcinoma
Guo-Hai Zhao, Tie-Chen Li, Liang-Hui Shi, Ya-Bin Xia, Lin-Ming Lu, Wen-Bin Huang, Hui-Lan Sun, Yi-Sheng Zhang
Guo-Hai Zhao, Liang-Hui Shi, Ya-Bin Xia, Yi-Sheng Zhang, Department of Surgery, the Affiliated Yijishan Hospital, Wannan Medical College, Wuhu 241001, Anhui Province, China
Tie-Chen Li, Hui-Lan Sun, Department of Biology, Wannan Medical College, Wuhu 241001, Anhui Province, China
Lin-Ming Lu, Wen-Bin Huang, Department of pathology, Wannan Medical College, Wuhu 241001, Anhui Province, China
Author contributions: All authors contributed equally to the work.
Supported by the Department of Education Natural Science Foundation of Anhui Province, China, No. 99jl0216
Correspondence to: Guo-Hai Zhao, Department of Surgery, the Affiliated Yijishan Hospital, Wannan Medical College, Wuhu 241001, Anhui Province, China. zhaoguohai@163.net
Telephone: +86-553-5738856-2107 Fax: +86-553-5738279
Received: October 9, 2002
Revised: October 23, 2002
Accepted: January 3, 2003
Published online: May 15, 2003
Abstract

AIM: To investigate the relationship between inactivation of p16 gene and gastric carcinoma, and the mechanism of inactivation of p16 gene in gastric carcinogenesis.

METHODS: 40 fresh tumor tissue specimens were taken from primary gastric cancer patients. Expression of p16 protein was detected by immunohistochemical method. Deletion and point mutation of p16 gene were analyzed by polymerase chain reaction (PCR) and DNA sequencing, respectively.

RESULTS: The frequency of loss of p16 protein expression in the gastric cancer tissue, adjacent nontumor tissue, and distal normal tissue was 77.5% (31/40), 55.0% (22/40), and 17.5% (7/40), respectively (P < 0.005). Homozygous deletion of exon 1 and exon 3 was observed in two and three cases, respectively, giving an overall frequency of homozygous deletion of 12.5%. All five cases had diffuse type gastric carcinoma. No p16 gene point mutation was detected.

CONCLUSION: These findings suggest a close correlation between inactivation of p16 gene and gastric carcinoma. Further investigations are needed to testify the mechanism of inactivation of p16 gene in gastric carcinogenesis.

Keywords: $[Keywords]