Basic Research
Copyright ©The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. May 15, 2003; 9(5): 1072-1076
Published online May 15, 2003. doi: 10.3748/wjg.v9.i5.1072
Modulation of GdCl3 and Angelica Sinensis polysaccharides on differentially expressed genes in liver of hepatic immunological injury mice by cDNA microarray
Hong Ding, Gang-Gang Shi, Xin Yu, Jie-Ping Yu, Jie-An Huang
Hong Ding, Gang-Gang Shi, Medical College, Shantou University, Shantou, 515031, Guangdong Province, China
Xin Yu, College of Pharmacy, Wuhan University, Wuhan, 430072, Hubei Province, China
Jie-Ping Yu, Jie-An Huang, Department of Gastroenterology, The First Affiliated Hospital, Clinical Medical College, Wuhan University, Wuhan, 430064, Hubei Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Hong Ding, College of Pharmacy, Wuhan University, Wuhan 430072, Hubei Province, China. dinghong2000@263.net
Telephone: +86-27-87682339 Fax: +86-27-87682339
Received: September 13, 2002
Revised: November 11, 2002
Accepted: November 28, 2002
Published online: May 15, 2003
Abstract

AIM: To study the modulating effect of GdCl3 and Angelica Sinensis polysaccharides (ASP) on differentially expressed genes in liver of hepatic immunological mice by cDNA microarray.

METHODS: Hepatic immunological injury was induced by lipopolysaccharide (LPS ip, 0.2 mg·kg-1) in bacillus calmetteguerin (BCG ip, 1 mg·kg-1) primed mice; A single dose of 20 mg·kg-1 GdCl3 was simultaneously pretreated and 30 mg·kg-1 ASP (ig, qd × 7 d) was administrated when the BCG+LPS was primed. The mice were sacrificed at the end of the 7th day after ip LPS for 6 h and the liver was removed quickly. The PCR products of 512 genes were spotted onto a chemical material-coated glass plate in array. The DNAs were fixed to the glass plate after series of treatments. The total RNAs were isolated from the liver tissue, and were purified to mRNAs by Oligotex. Both mRNAs from the normal liver tissue and the liver tissue from the mice with hepatic immunological injury or that pretreated with GdCl3 or ASP were reversely transcribed to cDNAs with the incorporation of fluorescent dUTP to prepare the hybridization probes. The mixed probes were hybridized to the cDNA microarray. After high-stringent washing, the cDNA microarray was scanned for fluorescent signals and showed differences between the two tissues.

RESULTS: Among the 512 target genes, 18 differed in liver tissue of hepatic immunological injury mice, and 6 differed in those pretreated by ASP, 7 differed in those pretreated by GdCl3.

CONCLUSION: cDNA microarray technique is effective in screening the differentially expressed genes between two different kinds of tissue. Further analysis of those obtained genes will be helpful to understand the molecular mechanism of hepatic immunological injury and to study the intervention of drug. Both ASP and GdCl3 can decrease the number of the differentially expressed genes in liver tissue of mice with hepatic immunological injury.

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