H.Pylori
Copyright ©The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 15, 2003; 9(2): 309-311
Published online Feb 15, 2003. doi: 10.3748/wjg.v9.i2.309
Evaluation of the string test for the detection of Helicobacter pylori
Rupert WL Leong, Ching C Lee, Thomas KW Ling, Wai K Leung, Joseph JY Sung
Rupert WL Leong, Department of Gastroenterology, University of New South Wales, Bankstown Hospital, Sydney, Australia
Ching C Lee, Thomas KW Ling, Department of Microbiology; Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong, China
Wai K Leung, Joseph JY Sung, Department of Medicine & Therapeutics, Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Professor Joseph JY Sung, Department of Medicine and Therapeutics, Prince of Wales Hospital, Shatin, N. T., Hong Kong, China. joesung@cuhk.edu.hk
Telephone: +852-26323132 Fax: +852-26467824
Received: August 24, 2002
Revised: September 4, 2002
Accepted: November 4, 2002
Published online: February 15, 2003
Abstract

AIM: Helicobacter pylori can be diagnosed by invasive or non-invasive tests but to obtain bacteria for culture and antibiotic susceptibility testing, an upper GI endoscopy is often required. The string test may be a minimally-invasive alternative method of obtaining H. pylori samples. This study evaluates the sensitivity and specificity of the string test in the diagnosis of H. pylori in comparison with endoscopic means of diagnosis.

METHODS: This was a prospective open comparative study of patients with dyspepsia with endoscopy-based tests as gold standard (defined as a positive CLO test and antral histology). Fasting patients swallowed the encapsulated-string (Entero-test Hp), which was withdrawn after 1 h. The gastric juice from the string was plated onto H. pylori-selective media for culture. Helicobacter pylori was identified by typical colony morphology, gram stain and biochemical test results.

RESULTS: Thirty dyspeptic patients were recruited of whom 21 (70%) were positive for H. pylori according to the gold standard. The sensitivity, specificity, positive predictive value, and negative predictive value for the string test were 38%, 100%, 100% and 41% respectively, and for endoscopic biopsies 81%, 100%, 100%, 69% respectively (P = 0.004). Logistic regression showed that only abundant growth density from endoscopic biopsy cultures to be a predictor of a positive string test (P = 0.018).

CONCLUSION: The string test is an alternative method to endoscopy in obtaining H. pylori but has a low sensitivity compared to endoscopic biopsies.

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