Published online Nov 15, 2003. doi: 10.3748/wjg.v9.i11.2596
Revised: July 14, 2003
Accepted: July 24, 2003
Published online: November 15, 2003
AIM: To detect the vascular endothelial growth factor (VEGF) and soluble splice variant 6 of CD44 (sCD44v6) levels in ascites and to explore their role in differentiating benign from malignant ascites.
METHODS: Cirrhotic ascites (n = 36), tuberculosis ascites (n = 8) and malignant ascites (n = 23) were collected and studied. Concentrations of soluble VEGF and sCD44v6 in various kinds of ascites (n = 67) were measured using a sandwich enzyme-linked immunoadsorbent assay.
RESULTS: VEGF and sCD44v6 levels in malignant ascites were 640.74 ± 264.81 pg/mL and 89.22 ± 38.20 ng/mL, respectively, both of which were significantly higher than those in cirrhotic ascites and tuberculous ascites (q = 18.98, 11.89 and q = 8.92, 5.09; P < 0.01). However, the levels of VEGF and sCD44v6 in cirrhotic and tuberculous ascites had no significant difference (q = 0.48, 0.75; P > 0.05). Furthermore, VEGF levels in malignant ascites in patients with ovarian cancer were higher than those with gastric and colon cancer (q = 5.03, 6.79; P < 0.01, respectively). But differences of VEGF levels between gastric and colon cancer were not significant (q = 1.90, P > 0.05). Whereas, sCD44v6 levels in malignant ascites from patients with ovarian, gastric and colon cancer had no significant difference (q = 0.06, 0.91, 0.35; P > 0.05, respectirely). In comparison with cirrhotic and tuberculous ascites, when the upper limit of its VEGF mean levels 119.44 pg/mL (70.90 ± 48.54) and sCD44v6 mean levels 63.59 ng/mL (44.42 ± 19.17) was taken as the minimum cutoff limit, the sensitivity and specificity of VEGF and sCD44v6 of this assay to the diagnos is of malignant ascites were 91.3%, 90.9% and 73.9%, 88.7% respectively.
CONCLUSION: Elevated levels of VEGF and sCD44v6 may be useful in differential diagnosis of benign and malignant ascites.