Hepatitis C virus infection of human hepatoma cell line 7721 in vitro

doi:10.3748/wjg.v7.i5.685

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Oct 15, 2001 (publication date) through Feb 21, 2025

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Oct 15, 2001; 7(5): 685-689
Published online Oct 15, 2001. doi: 10.3748/wjg.v7.i5.685

Hepatitis C virus infection of human hepatoma cell line 7721 in vitro

Zhi Qiang Song, Fei Hao, Feng Min, Qiao Yu Ma, Guo Dong Liu

Zhi Qiang Song, Fei Hao, Department of Dermatology, Third Military Medical University, Chongqing 400038, China

Feng Min, Qiao Yu Ma, Guo Dong Liu, Department of Infectious Diseases, Southwest Hospital, Third Military Medical University, Chongqing 400038, China

Author contributions: All authors contributed equally to the work.

Supported by the National Natural Science Foundation of China, No.39670672

Correspondence to: Dr. Zhi Qiang Song, Department of Dermatology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.songzq@yeah.net.

Telephone: +86-23-68753298

Received: January 3, 2001
Revised: January 6, 2001
Accepted: February 12, 2001
Published online: October 15, 2001

Abstract

AIM: To establish a cell culture system with long-term replication of hepatitis C virus in vitro.

METHODS: Human hepatoma cell line 7721 was tested for its susceptibility to HCV by incubating with a serum from a patient with chronic hepatitis C. Cells and supernatant were harvested at various phases during the culturing periods. The presence of HCV RNA, the expression of HCV antigens in cells and/or supernatant were examined by RT-PCR, in situ hybridization and immunohisto-chemistry respectively.

RESULTS: The intracellular HCV RNA was first detected on d2 after infection and then could be intermittently detected in both cells and supernatant over a period of at least three months. The expression of HCV NS₃, CP₁₀ antigens could be observed in cells. The fresh cells could be infected by supernatant from cultured infected cells and the transmission of viral genome from HCV-infected 7721 cells to PBMCs was also observed.

CONCLUSION: The hepatoma line 7721 is not only susceptible to HCV but also supports its long-term replication in vitro.

Keywords: hepatitis C virus; cell culture; cell model; carcinoma, hepatocellular/pathology; tumor cells, cultured; hepatitis B virus; virus replication