Abstracts
Copyright ©The Author(s) 2000. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 15, 2000; 6(Suppl3): 88-88
Published online Sep 15, 2000. doi: 10.3748/wjg.v6.iSuppl3.88
Dynamic observation of T cell and erythrocyte immune function in children with rotavirus enteritis
Fang Liu, De He, Hong Xiao
Fang Liu, De He, Department of Microbiology, Guangdong Medical College, Zhanjiang 524023, Guangdong Province, China
Hong Xiao, Department of Pediatrics, the Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, Guangdong Province, China
Author contributions: All authors contributed equally to the work.
Supported by The Scientific Foundation of Guangdong Medical College, No. 9404
Correspondence to: Dr. Fang Liu, Department of Microbiology, Guangdong Medical College, 2 Wenming Donglu, Xiashan, Zhanjiang 524023, Guangdong Province, China. gdmcwsw@gdmc.edu.cn
Telephone: 759-2283633 Fax: 759-2284104
Received: December 22, 1999
Revised: April 20, 2000
Accepted: July 10, 2000
Published online: September 15, 2000
Abstract

AIM: To study the change of T cell subsets and erythrocytes immune function in children with acute rotavirus enteritis (RE) and to explore its clinical significance.

METHODS: T cell subsets were detected by indirect immunofluorescence technique, RBC immune function was achieved by detecting RBC C3b receptor rosette (RBC-CR1R) and RBC immune complex rosette (RBC-ICR). The statistic al significance of differences was evaluated by using analysis of variance (ANOVA).

RESULTS: The percentage rate of CD3 and CD4 cell and the ratio of CD4/CD8 in acute stage of RE were significantly lower than those of control (P < 0.01), while the percentage rate of CD8 cell remained almost unchanged. Along with the improvement of clinical symptoms, the level of CD3, CD4 and CD4/CD8 returned to normal in recovery stage. RBC-CR1R in acute stage of RE was significantly lower than that of control (F = 15.44, P < 0.01) and returned to normal in recovery stage. RBC-ICR rose slightly in acute stage of RE, but there was no significant difference between acute stage of RE and the control (F = 0.02518, P > 0.05). Correlative analysis showed that RBC-CR1R was positively correlated to the percentage rate of CD4 cell (r = 0.5281, P < 0.01) and the ratio of CD4/CD8 (r = 0.4832, P < 0.01), in acute stage of RE.

CONCLUSION: Cellular immune might take part in the development of RE, CD4/CD8 ratio might be related to the prognosis of RE. The immune function of RBC reduced secondarily in acute stage of RE. It is necessary to keep the immune function of RBC in treatment of RE in order to strengthen the patient’s ability of anti-infection.

Keywords: Rotavirus enteritis/in infancy and children; Rotavirus infection/in infancy and children; T-lymphocyte subsets; Erythrocytes/immunology; Fluorescent antibody technique; Analysis, variance