Effect of boschniakia rossica on expression of GST-P, p53 and p21ras proteins in early-stage chemical hepatocarcinogenesis and its anti-inflammatory activities in rats

doi:10.3748/wjg.v6.iSuppl3.84

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 6, Issue Suppl3

This Article

Citation of this article

Corresponding Author of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (548)

All Articles published online

The chart showing PDF series, HTML series.

Item

Count

PDF

HTML

467

Sum=548

Sep 15, 2000 (publication date) through Dec 26, 2024

Times Cited of This Article

Times Cited (0)

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Abstracts

World J Gastroenterol. Sep 15, 2000; 6(Suppl3): 84-84
Published online Sep 15, 2000. doi: 10.3748/wjg.v6.iSuppl3.84

Effect of boschniakia rossica on expression of GST-P, p53 and p21^ras proteins in early-stage chemical hepatocarcinogenesis and its anti-inflammatory activities in rats

Zong-Zhu Yin, Hai-Ling Jin, Xue-Zhe Yin, Tian-Zhu Li, Ji-Shu Quan, Zeng-Nan Jin

Zong-Zhu Yin, Hai-Ling Jin, Xue-Zhe Yin, Tian-Zhu Li, Ji-Shu Quan, Zeng-Nan Jin, Institute for Cancer Research, Yanbian University College of Medicine, Yanji 133000, Jilin Province, China

Author contributions: All authors contributed equally to the work.

Supported by The National Natural Science Foundation of China, No. 39660021

Correspondence to: Dr. Zong-Zhu Yin, Professor, Institute for Cancer Research, Yanbian University College of Medicine, 121 Juzi Street, Yanji 133000, Jilin Province, China. yinzz@public.yj.jl.cn

Telephone: 433-2660589 Fax: 433-2621142

Received: April 24, 2000
Revised: May 20, 2000
Accepted: June 10, 2000
Published online: September 15, 2000

Abstract

AIM: To investigate the effect of boschniakia rossica (BR) extract on expression of GST-P, p53 and p21^ras proteins in early stage chemical hepatocarcinogenesis in rats and its anti-inflammatory actions.

METHODS: The expression of tumor marker, placental form glut athione S-transferase (GST-P), p53 and p21^ras proteins were investigated by immunohistochemical techniques and ABC method. Anti-inflammatory activities of BR were observed by xylene and croton oil-induced mouse ear edema, carrageenin, histamine and hot scald-induced rat pow edema, adjuvant-induced rat arthritis and cotton pellet induced mouse granuloma formation methods.

RESULTS: The 500 mg/kg of BR-H₂O extract fractionated from BR Methanol extract had inhibitory effect on the formation of DEN-induced GST-P-positive foci in rat liver and the expression of mutant p53 and p21^ras protein was lower than that of hepatic preneoplastic lesions. Both CH₂Cl₂ and H₂O extract from BR have inhibitory effect in xylene and croton oil-induced mouse ear edema. BR-H₂O extract exhibited inhibitory effect in carrageenin, histamine and hot scald-induced hind paw edema and adjuvant-induced arthritis in rats and cotton pellet induced granuloma formation in mice.

CONCLUSION: BR extract exhibited inhibitory effect on formation of Preneoplastic hepatic foci in early stage of rat chemical hepatocarcinogenesis. Both CH₂Cl₂ and H₂O extract from BR exerted anti-inflammatory effect in rats and mice.

Keywords: Boschniakia rossica; Liver neoplasms/chemically induced; Glutathione transferases; Protein p53; Proto-oncogene protein p21 (Ras); Immunohistochemistry; Arthritis, adjuvant; Antibiotics