Effect of boschniakia rossica on expression of GST-P, p53 and p21ras proteins in early-stage chemical hepatocarcinogenesis and its anti-inflammatory activities in rats

doi:10.3748/wjg.v6.iSuppl3.84

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Sep 15, 2000 (publication date) through Nov 20, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Sep 15, 2000; 6(Suppl3): 84-84
Published online Sep 15, 2000. doi: 10.3748/wjg.v6.iSuppl3.84

Effect of boschniakia rossica on expression of GST-P, p53 and p21^ras proteins in early-stage chemical hepatocarcinogenesis and its anti-inflammatory activities in rats

Zong-Zhu Yin, Hai-Ling Jin, Xue-Zhe Yin, Tian-Zhu Li, Ji-Shu Quan, Zeng-Nan Jin

Zong-Zhu Yin, Hai-Ling Jin, Xue-Zhe Yin, Tian-Zhu Li, Ji-Shu Quan, Zeng-Nan Jin, Institute for Cancer Research, Yanbian University College of Medicine, Yanji 133000, Jilin Province, China

Author contributions: All authors contributed equally to the work.

Supported by The National Natural Science Foundation of China, No. 39660021

Correspondence to: Dr. Zong-Zhu Yin, Professor, Institute for Cancer Research, Yanbian University College of Medicine, 121 Juzi Street, Yanji 133000, Jilin Province, China. yinzz@public.yj.jl.cn

Telephone: 433-2660589 Fax: 433-2621142

Received: April 24, 2000
Revised: May 20, 2000
Accepted: June 10, 2000
Published online: September 15, 2000

Abstract

AIM: To investigate the effect of boschniakia rossica (BR) extract on expression of GST-P, p53 and p21^ras proteins in early stage chemical hepatocarcinogenesis in rats and its anti-inflammatory actions.

METHODS: The expression of tumor marker, placental form glut athione S-transferase (GST-P), p53 and p21^ras proteins were investigated by immunohistochemical techniques and ABC method. Anti-inflammatory activities of BR were observed by xylene and croton oil-induced mouse ear edema, carrageenin, histamine and hot scald-induced rat pow edema, adjuvant-induced rat arthritis and cotton pellet induced mouse granuloma formation methods.

RESULTS: The 500 mg/kg of BR-H₂O extract fractionated from BR Methanol extract had inhibitory effect on the formation of DEN-induced GST-P-positive foci in rat liver and the expression of mutant p53 and p21^ras protein was lower than that of hepatic preneoplastic lesions. Both CH₂Cl₂ and H₂O extract from BR have inhibitory effect in xylene and croton oil-induced mouse ear edema. BR-H₂O extract exhibited inhibitory effect in carrageenin, histamine and hot scald-induced hind paw edema and adjuvant-induced arthritis in rats and cotton pellet induced granuloma formation in mice.

CONCLUSION: BR extract exhibited inhibitory effect on formation of Preneoplastic hepatic foci in early stage of rat chemical hepatocarcinogenesis. Both CH₂Cl₂ and H₂O extract from BR exerted anti-inflammatory effect in rats and mice.

Keywords: Boschniakia rossica; Liver neoplasms/chemically induced; Glutathione transferases; Protein p53; Proto-oncogene protein p21 (Ras); Immunohistochemistry; Arthritis, adjuvant; Antibiotics