Abstracts
Copyright ©The Author(s) 2000. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 15, 2000; 6(Suppl3): 70-70
Published online Sep 15, 2000. doi: 10.3748/wjg.v6.iSuppl3.70
Effects of sinusoidal endothelial cell conditioned medium on the expression of connective tissue growth factor in rat hepatic stellate cells
Xiao-Jing Liu, Fang Liu, Wen-Jun Xiao, Ming-Hui Huang, Song-Min Huang, Yi-Ping Wang
Xiao-Jing Liu, Ming-Hui Huang, Laboratory of Department of Internal Medicine of the First University Hospital, West China University of Medical Sciences, Chengdu 610041, Sichuan Province, China
Fang Liu, Wen-Jun Xiao, Song-Min Huang, Yi-Ping Wang, Department of Internal Medicine of the First University Hospital, West China University of Medical Sciences, Chengdu 610041, Sichuan Province, China
Author contributions: All authors contributed equally to the work.
Supported by The National Natural Science Foundation of China, No.39700068
Correspondence to: Dr. Xiao-Jing Liu, Laboratory of Department of Internal Medicine, the First Hospital of West China University of Medical Sciences, 37 Wainan Guoxuexiang, Chengdu 610041, Sichuan Province, China. xiaojing66@ hotmail.com
Telephone: 28-5422388
Received: May 5, 2000
Revised: June 10, 2000
Accepted: July 10, 2000
Published online: September 15, 2000
Abstract

AIM: To investigate the effects of sinusoidal endothelial cell (SEC) conditioned medium on the expression of connective tissue growth factor (CTGF) in rat hepatic stellate cells (HSC).

METHODS: By in situ collagenase perfusion and two-step Percoll gradient centrifugation, SECs were isolated and cultured from normally and CCl4 treated Wistar rats, and the SEC conditioned media were collected. HSCs were prepared from Wistar rats by in situ perfusion and single step Nycodenzgradient, and were cultured with SEC conditioned media. Expression of CTGF in HSC was assessed using reverse transcription polymerase chain reaction (RT-PCR).

RESULTS: Expression of CTGF was not found in freshly isolated HSC and in primary culture of HSC on day 4 with SEC conditioned media from normal rats, but was present in primary culture of HSC on day 4 with SEC conditioned media from CCl4 induced liver fibrosis rats. Expression of CTGF was observed in culture-activated HSCs, and the effect of SEC conditioned media from CCl4 induced liver fibrosis rats on the expression of CTGF gene in activated HSCs was not significant.

CONCLUSION: Expression of CTGF might be relative to the activation of HSC and the liver fibrogenesis, and damaged SECs play a very important role in the early stage of activation of HSC.

Keywords: Sinusoidal endothelial cell; Hepatic stellate cell; Connective tissue growth factor; Culture media; Clostridium histolyticum collagenase; Polymerase chain reaction