Effect of TNF gene-transfected LAK cells on the ascitic liver carcinoma-bearing mice

doi:10.3748/wjg.v6.iSuppl3.19

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Sep 15, 2000; 6(Suppl3): 19-19
Published online Sep 15, 2000. doi: 10.3748/wjg.v6.iSuppl3.19

Effect of TNF gene-transfected LAK cells on the ascitic liver carcinoma-bearing mice

Guo-Liang Lou, Xue-Tao Cao, Bi-He Min, Wei-Ping Zhang, Pei-Lin Meng

Guo-Liang Lou, Bi-He Min, Pei-Lin Meng, Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China

Xue-Tao Cao, Wei-Ping Zhang, Department of Immunology, Second Military Medical University, Shanghai 200433, China

Author contributions: All authors contributed equally to the work.

Supported by the National Natural Science Foundation of China, No. 39421009

Correspondence to: Dr. Guo-Liang Lou, Department of Hematology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China

Telephone: 21-25070705

Received: May 23, 1999
Revised: June 28, 2000
Accepted: July 10, 2000
Published online: September 15, 2000

Abstract

AIM: To investigate the therapeutic effect of TNF gene transfected LAK cells on ascitic liver carcinoma bearing mice.

METHODS: TNF gene was transfected into murine LAK cells by retrovirus. Low dose TNF gene transfected LAK cells and IL-2 were i.p. injected into murine model. Cytotoxicity of gene transfected LAK cells was studied in vitro growth and the survival time of murine model was observed.

RESULTS: TNF gene-transfected LAK cells secreted higher level of TNF than that of normal LAK cells or control gene-transfected LAK cells. The in vitro growth ability and cytotoxicity of TNF gene-transfected LAK cells were markedly inhibited by anti-TNF monoclonal antibodies. Significant therapeutic effect on ascitic liver carcinoma bearing mice was achieved.

CONCLUSION: TNF gene transfected LAK cells have therapeutic effect on ascitic liver carcinoma bearing mice.

Keywords: Tumor necrosis factor; Kill cells, lymphokine-activated; Live neoplasms/therapy; Gene therapy; Transfection; Mice