Abstracts
Copyright ©The Author(s) 2000. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 15, 2000; 6(Suppl3): 100-100
Published online Sep 15, 2000. doi: 10.3748/wjg.v6.iSuppl3.100
Study on the dynamic alterations of serum HA in rats with carbon Tetrachloride-induced liver fibrosis
Lan Ma, Li-Sheng Zhao, Chun-Hua Li, Qi Lu, Ren-Kuan Li, Shuang-Seng Deng
Lan Ma, Li-Sheng Zhao, Chun-Hua Li, Qi Lu, Ren-Kuan Li, Shuang-Seng Deng, Monoclonal Antibody Biotechnology Center of Yunnan University, Kunming 650091, Yunnan Province, China
Author contributions: All authors contributed equally to the work.
Supported by The Natural Scientific Foundation of Yunnan Province, No. 96C010M
Correspondence to: Dr. Lan Ma, Monoclonal Antibody Biotechnology Center of Yunnan University, Kunming 650091, Yunnan Province, China. lisheng@public.km.yn.cn
Telephone: 871-5033160 Fax: 871-5033626
Received: May 5, 2000
Revised: June 10, 2000
Accepted: July 10, 2000
Published online: September 15, 2000
Abstract

AIM: To study the clinical significance of alterations of serum hyaluronic acid in rats with carbon tetrachloride induced liver fibrosis.

METHODS: Rat liver fibrosis model was induced by carbon tetrachloride (CCl4). The rats were divided into five groups: group 1 (control): 0 wk with no CCl4-inducing; group 2, 3, 4 and 5: 3, 6, 9 and 12 wk after CCl4-induction respectively. Serum HA level was analysed among various live rfibrosis groups and control, and then compared the HA findings with the hepatic histopathology.

RESULTS: During rat liver fibrosis, serum HA levels of the liver fibrosis groups (group 2: 7.98 ng/mL; group 3: 20.10 ng/mL; group 4: 229.73 ng/mL; group 5: 324.74 ng/mL) were significantly higher than that of control group (group 1: 0.21 ng/mL) (P < 0.01), in which group 4 and group 5 are much higher 1094 times (229.73 ng/mL/0.21 ng/mL)and 1546 times (324.74 ng/mL/0.21 ng/mL) than group 1 respectively. When compared with each other, the serum HA levels are 38 times (7.98 ng/mL/0.21 ng/mL; P < 0.01, group 2 vs group 1); 2.5 times (20.10 ng/mL/7.98 ng/mL; P < 0.01, group 3 vs group 2); 11.4 times (229.73 ng/mL/20.10 ng/mL; P < 0.01, group 4 vs group 3); 1.4 times (324.74 ng/mL/229.73 ng/mL; P < 0.01, group 5 vs group 4) respectively.

CONCLUSION: The results demonstrated that the dynamic alterations of serum HA play an important role in the early clinical diagnosis and staging of liver cirrhosis.

Keywords: Hyaluronic acid/blood; Liver fibrosis; Rats; Sandwich immunoassay; Carbon tetrachloride; Immunohistochemistry