Wu P, Li X, Zhou T, Zhang MJ, Chen JL, Wang WM, Chen N, Dong DC. Role of P-selectin and anti-P-selectin monoclonal antibody in apoptosis during hepatic/renal ischemia-reperfusion injury. World J Gastroenterol 2000; 6(2): 244-247 [PMID: 11819566 DOI: 10.3748/wjg.v6.i2.244]
Corresponding Author of This Article
Dr. Tong Zhou, Department of Nephrology, Ruijin Hospital, Shanghai Second Medical University, 197 Ruijin Erlu, Shanghai 200025, China
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World J Gastroenterol. Apr 15, 2000; 6(2): 244-247 Published online Apr 15, 2000. doi: 10.3748/wjg.v6.i2.244
Role of P-selectin and anti-P-selectin monoclonal antibody in apoptosis during hepatic/renal ischemia-reperfusion injury
Pei Wu, Xiao Li, Tong Zhou, Ming Jun Zhang, Jin Lian Chen, Wei Ming Wang, Nan Chen, De Chang Dong
Pei Wu, Xiao Li, Tong Zhou, Wei Ming Wang, Nan Chen, De Chang Dong Department of Nephrology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China;
Ming Jun Zhang, Animal Laboratory, Ruijin Hospital, Shanghai Second Medical University;
Jin Lian Chen, Department of Gastroenterology, Ruijin Hospital, Shanghai Second Medical University;
Pei Wu, earned master degree from Shanghai Second Medical University in 1997, doctor in charge of nephrology, majoring the role of cell adhesion molecules in renal diseases, having 6 papers published.
Author contributions: All authors contributed equally to the work.
Supported by the Scientific Foundation of Ministry of Health of China, No. 98-2-283 and Shanghai Natural Science Foundation, No. 98ZB14025
Correspondence to: Dr. Tong Zhou, Department of Nephrology, Ruijin Hospital, Shanghai Second Medical University, 197 Ruijin Erlu, Shanghai 200025, China
Received: July 1, 1999 Revised: November 6, 1999 Accepted: November 19, 1999 Published online: April 15, 2000
Abstract
AIM: To evaluale the potential role of P-selectin and anti-P-selectin monoclonal antibody (mAb) in apoptosis during -hepatic/renal ischemia-reperfusion injury.
METHODS: Plasma P-selectin level, hepatic/renal P-selectin expression and cell apoptosis were detected in rat model of hepatic/renal ischemia-reperfusion injury. ELISA, immunohist-ochemistry and TUNEL were used. Some ischemia-reperfusion rats were treated with anti-P-selectin mAb.
RESULTS: Hepatic/renal function insuffic-iency, up-regul ated expression of P-selectin in plasma and hepatic/renal tissue, hepatic/renal histopathological damages and cell apoptosis were found in rats with hepatic/renal ischemia-reperfusion injury, while these changes became less conspicuous in animals treated with anti-P-selectin mAb.
CONCLUSION: P-selectin might mediate neutrophil infiltration and cell apoptosis and contribute to hepatic/renal ischemia-reperfusion injury, anti-P-selectin mAb might be an efficient approach for the prevention and treatment of hepatic/renal ischemia-reperfusion injury.
