Original Articles
Copyright ©The Author(s) 2000. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 15, 2000; 6(1): 111-114
Published online Feb 15, 2000. doi: 10.3748/wjg.v6.i1.111
Relationship of lmp2 and DR3 genes with susceptibility to type I diabetes mellitus in south China Han population
He-Lin Ding, Hua Cheng, Zu-Zhi Fu, Qing-Li Deng, Li Yan, Tang Yan
He-Lin Ding, Hua Cheng, Zu-Zhi Fu, Qing-Li Deng, Li Yan, Tang Yan, Department of Endocrinology, Sun Yat-Sen Memorial Hospital, Sun Yat Sen University of Medical Sciences, Guangzhou 510120, China
He-Lin Ding, male, born on April 18, 1963 in Feidong County, Anhui Prov ince, got master degree of medicine from Sun Yat-Sen University of Medical Scie nces in 1991. Now working as lecturer of medicine, and had 7 papers published.
Author contributions: All authors contributed equally to the work.
Correspondence to: He-Lin Ding, Department of Endocrinology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University of Medical Sciences, 107 Yanjiang West Road, Guangzhou 510120, Guangdong Province, China
Received: July 22, 1999
Revised: September 22, 1999
Accepted: October 11, 1999
Published online: February 15, 2000
Abstract

AIM: To study the relationship of lmp2 and DR3 genes with type I diabetes mellitus.

METHODS: lmp2 genotypes and DR3 were identified in 68 patien ts with type I diabetes mellitus (I-DM) and 71 healthy controls. Then, I-D M patients and controls were respectively allocated into DR3-positive and DR3- negative groups. The frequencies of lmp2 and DR3 gene in random subjects, an d lmp2 genotypes in DR3-matched subjects were compared between I-DM patie nts and controls. At the same time, I-DM patients were divided into 3 groups b ased on the onset age of diabetics: group A ≤ 14 years, group B 15-30 years and group C ≥ 31 years.

RESULTS: The frequency of DR3 in I-DM patients was significantly higher than that in controls (47% vs 21%, P < 0.005), and it was significantly higher in group A than that in group B + C (70% vs 36%, χ2 = 7.07, P < 0.01). There was a significant difference among groups with different onset age of diabetics (χ2 = 8.19, rp = 0.33, P < 0.05). In random subjects, the frequency of lmp2 R/R in I-DM patients was lower (43% vs 61%, P < 0.05) and lmp2-R/H higher (53% vs 28%, P < 0.05) than that in controls, and there was no significant difference among groups with different onset age of diabetics. In DR3-positive subjects, the frequency of lmp2-R/R in I-DM patients was lower (47% vs 87%, P < 0.05) and lmp2- R/H higher (47% vs 13%, P < 0.05) than that in controls. In DR3-negative subjects, the frequency of lmp2-R/H in I-DM patients was higher than that in controls (58% vs 32%, P < 0.01), but the frequency of lmp 2-R/R and lmp2-H/H was not significantly different between these two groups.

CONCLUSION: DR3 gene may be one of the susceptible genes of I-DM, and significantly related to the onset age of diabetics, and the persons with DR3 may have an younger onset age of diabetes. The lmp2-R/R may be the protective genotype of I-DM, and lmp2-R/H the susceptible genotype. These were not affected by DR3 gene. lmp2 genotypes were not related with the onset age of diabetics.

Keywords: diabetes mellitus; Imp2 genes; DR3 genes; polymerase chain reaction; restriction fragment length polymorphism; genetic susceptibility