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©The Author(s) 1999. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jun 15, 1999; 5(3): 241-244
Published online Jun 15, 1999. doi: 10.3748/wjg.v5.i3.241
Published online Jun 15, 1999. doi: 10.3748/wjg.v5.i3.241
Cloning and identification of an angiostatic molecule IP-10/crg-2
Zhi-Guo Liu, Hua-Zhang An, Hai-Yan Wang, Feng-Tian He, Zhe-Yi Han, Ying Han, Han-Ping Wu, Bing Xiao, Dai-Ming Fan, Department of Gastroenterology, Xijing Hospital, the Fourth Military Medical University, Xi’an 710032, China
Jing-Hua Yang, Department of Cellular and Molecular Biology, Harvard University, USA
Zhi-Guo Liu, male, born on 1974-03-24 in Harbin of China, graduat ed from Department of Medicine, the Fourth Military Medical University (FMMU) with bachelor degree of medicine in 1997, and now as a postgraduate in Department of Gastroenterology of Xijing Hospital, FMMU.
Author contributions: All authors contributed equally to the work.
Supported by the Outstanding Youth Fund from National Natural Sc ience Foundation of China, No. 39625023.
Correspondence to: Dr. Dai-Ming Fan, Department of Gastroentero logy, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China. zhiguoliu@163.net
Telephone: +86-29-2539041 Fax: +86-29-2539041
Received: December 3, 1998
Revised: March 1, 1999
Accepted: March 20, 1999
Published online: June 15, 1999
Revised: March 1, 1999
Accepted: March 20, 1999
Published online: June 15, 1999
Abstract
AIM: To obtain human and murine cDNAs encoding IFN-γ inducible protein 10 (IP-10) and cytokine responsive gene-2 (Crg-2).
METHODS: The encoding genes of IP-10 and Crg-2 were amplified by RT-PCR from cultured human fibroblast cells and Balb/c mouse liver treated by IFN-γ and TNF-α , respectively, and cloned into plasmids of pUC19 and pGEM3Zf(+).
RESULTS: The nucleotide sequences of the amplified DNA were confirmed by endonucleases digestion and sequencing.
CONCLUSION: Recombinant IP-10/crg-2 gene clones with 306 bp and 314 bp inserts were established for further research on biological activities and ligands of hIP-10/mCrg-2
Keywords: IFN-α, IFN-γ, inducible protein, cytokine vesponsive gene-2, DNA