Combined Traditional and Modern Medicine
Copyright ©The Author(s) 1998. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 15, 1998; 4(6): 548-549
Published online Dec 15, 1998. doi: 10.3748/wjg.v4.i6.548
Effects of Fuzhenghuayu decoction on collagen synthesis of cultured hepatic stellate cells, hepatocytes and fibroblasts in rats
Cheng Liu, Ping Liu, Cheng-Hai Liu, Xiu-Qing Zhu, Guang Ji
Cheng Liu, Ping Liu, Cheng-Hai Liu, Xiu-Qing Zhu, Guang Ji, Institute of Liver Disease, Shanghia University of Traditional Chinese Medicine, Shanghai 200032, China
Cheng Liu, M.D., male, born on 1939-03-03 in Shanghai, graduated from Shanghai Medical University in 1962, now as professor of medicine and tutor for doctoral students, having 60 papers and 5 books published.
Author contributions: All authors contributed equally to the work.
Correspondence to: Prof. Cheng Liu, Institute of Liver Disease, Shanghai University of Traditional Chinese Medicine, 503 Lingling Rd, Shanghai, 200032, China
Telephone: +86-21-64036889
Received: July 16, 1998
Revised: September 20, 1998
Accepted: November 24, 1998
Published online: December 15, 1998
Abstract

AIM: To study the mechanism of Fuzhenghuayu (FZHY) decoction on anti-liver fibrosis.

METHODS: FZHY 10% decoction sera was incubated with rat normal subcultured hepatic stellate cells (HSC) and fibrotic primarily cultured HSC, normal and fibrotic hepatocytes and subcultured skin fibroblasts seperately. Cell intracellular and extracellular collagen synthesis rates were measured by the method of [3H] Proline impulse and collagenase digestion.

RESULTS: For primarily cultured HSC and hepatocytes, both of intracellular and extracellular collagen synthesis rates decreased in the drug sera group. For the normal subcultured HSC and primarily cultured hepatocytes, the extracellular collagen secretion was decreased obviously by the drug sera, and intracellular collagen synthesis rates were inhibited to some extents. For fibroblasts, both intracellular and extracellular collagen synthesis rates were inhibited somewhat, but no significant differences were found.

CONCLUSION: The mechanism of FZHY decoction on anti-liver fibrosis may be associated with inhibition of liver collagen production.

Keywords: Fuzhenghuayu decoction, collagen synthesis, hepatic stellate cells, hepatocytes, fibroblasts