Original Articles
Copyright ©The Author(s) 1998. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Oct 15, 1998; 4(5): 409-411
Published online Oct 15, 1998. doi: 10.3748/wjg.v4.i5.409
Liver metastasis models of human colorectal carcinoma established in nude mice by orthotopic transplantation and their biologic characteristic
Qiu-Zhen Liu, Chao-Wei Tuo, Bin Wang, Bing-Quan Wu, Yan-Hua Zhang
Qiu-Zhen Liu, Chao-Wei Tuo, Bin Wang, Yan-Hua Zhang, Department of Hepatobiliary Surgery, No.202 Hospital of PLA, Shenyang 110003, Liaoning Province, China
Bing-Quan Wu, Institute of Pathology, Beijing Medical University, Beijing 100069, China
Qiu-Zhen Liu, female, born on 1949-11-10 in Benxi City, Liaoning Province, graduated from the Second Military Medical University in 1973, director and associate professor of hepatobiliary surgery of No.202 Hospital of PLA, having 43 papers published and awarded the second class prize for military science progress twice.
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Qiu-Zhen Liu, Department of Hepatobiliary Surgery, No.202 Hospital of PLA, Shenyang 110003, Liaoning Province, China
Received: June 6, 1998
Revised: July 20, 1998
Accepted: August 16, 1998
Published online: October 15, 1998
Abstract

AIM: To establish a liver metastasis model of human colorectal carcinoma in nude mice. METHODS: Orthotopic transplantation of histologically intact colorectal tissues from patients into colorectal mucosa of nude mice. Tumorgenicity, invasion, metastasis and morphological characteristics of the transplanted tumors were studied by light microscopy, electron microscopy and immunohistochemistry.

RESULTS: Liver metastasis models of human colon carcinoma (HCA-HMN-1) and human rectal carcinoma (HRA-HMN-2) were established after sceening from 34 colorectal carcinomas.They had been passaged in vivo for 18 and 21 generations respectively. There were lymphatic, hemotogenous and implanting metastasesis. CEA secretion was maintained after transplantation. The primary and liver metastatic tumors were similar to the original human carcinoma in histopathological and ultrastructural features, DNA content and chromosomal karyotype.

CONCLUSION: The liver metastasis models provide useful tools for the study of mechanism of metastasis and its treatment of human colorectal cancer.

Keywords: colorectal neoplasms; liver neoplasms/secondary; neoplasms transplatation; neoplasms metastasis; disease models, animal