Hua JS, Khin MM, Zheng PY, Yeoh KG, Ng HC, Ho B. Serum IgG response to differentiated antigens of Helicobacter pylori. World J Gastroenterol 1998; 4(3): 249-251 [PMID: 11819288 DOI: 10.3748/wjg.v4.i3.249]
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Dr. Jie-Song Hua, Department of Microbiology, National University of Singapore, Singapore 119074, Republic of Singapore. michob@nus.edu.sg
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World J Gastroenterol. Jun 15, 1998; 4(3): 249-251 Published online Jun 15, 1998. doi: 10.3748/wjg.v4.i3.249
Serum IgG response to differentiated antigens of Helicobacter pylori
Jie-Song Hua, Mar Mar Khin, Peng-Yuan Zheng, Khay-Guan Yeoh, Han Chong Ng, Bow Ho
Jie-Song Hua, Mar Mar Khin, Peng-Yuan Zheng, Han Chong Ng, Bow Ho, Department of Microbiology, National University of Singapore, Lower Kent Ridge Road, Singapore 119074, Republic of Singapore
Khay-Guan Yeoh, Department of Medicine, National University of Singapore, Lower Kent Ridge Road, Singapore 119074, Republic of Singapore
Jie-Song Hua, male, born on 1961-11-16, in Shanghai, graduated from Shanghai Medical University as bachelor in 1985 and master in 1991. Having 20 papers published.
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Jie-Song Hua, Department of Microbiology, National University of Singapore, Singapore 119074, Republic of Singapore. michob@nus.edu.sg
Telephone: +65-8743285
Received: April 25, 1998 Revised: May 10, 1998 Accepted: May 20, 1998 Published online: June 15, 1998
Abstract
AIM: To detect antibodies against Helicobacter pylori spiral and coccoid antigens in human sera.
METHODS: Blood samples were collected from 278 patients with gastric diseases. A 3-day-old culture of H. pylori on chocolate blood agar was used to providespiral form. ‘Synchronous’ coccoids were cultured in (BHY) (brain heart infusion supplemented with 10% horse serum and 0.4% yeast extract) medium in a chemostat. Antigens from spiral and coccoid form were prepared using acid glycine extraction. Enzyme-linked immunosorbent assay (ELISA) was performed to detect serum IgG antibodies against spiral and coccoid forms of H. pylori.
RESULTS: Seroprevalence of H. pylori infection was higher in patients with gastric ulcer (79%) and gastric cancer (83%) than those with non-ulcer dyspepsia (NUD) (44%) and other diseases (45%) (P < 0.05). IgG antibodies against spiral and coccoid antigens were detected in 50.7% (141/278) and 49.6% (138/278), respectively.
CONCLUSION: The spiral and coccoid forms of H. pylori coexist in patients infected with the bacterium.
