Serum IgG response to differentiated antigens of Helicobacter pylori

doi:10.3748/wjg.v4.i3.249

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jun 15, 1998; 4(3): 249-251
Published online Jun 15, 1998. doi: 10.3748/wjg.v4.i3.249

Serum IgG response to differentiated antigens of Helicobacter pylori

Jie-Song Hua, Mar Mar Khin, Peng-Yuan Zheng, Khay-Guan Yeoh, Han Chong Ng, Bow Ho

Jie-Song Hua, Mar Mar Khin, Peng-Yuan Zheng, Han Chong Ng, Bow Ho, Department of Microbiology, National University of Singapore, Lower Kent Ridge Road, Singapore 119074, Republic of Singapore

Khay-Guan Yeoh, Department of Medicine, National University of Singapore, Lower Kent Ridge Road, Singapore 119074, Republic of Singapore

Jie-Song Hua, male, born on 1961-11-16, in Shanghai, graduated from Shanghai Medical University as bachelor in 1985 and master in 1991. Having 20 papers published.

Author contributions: All authors contributed equally to the work.

Correspondence to: Dr. Jie-Song Hua, Department of Microbiology, National University of Singapore, Singapore 119074, Republic of Singapore. michob@nus.edu.sg

Telephone: +65-8743285

Received: April 25, 1998
Revised: May 10, 1998
Accepted: May 20, 1998
Published online: June 15, 1998

Abstract

AIM: To detect antibodies against Helicobacter pylori spiral and coccoid antigens in human sera.

METHODS: Blood samples were collected from 278 patients with gastric diseases. A 3-day-old culture of H. pylori on chocolate blood agar was used to providespiral form. ‘Synchronous’ coccoids were cultured in (BHY) (brain heart infusion supplemented with 10% horse serum and 0.4% yeast extract) medium in a chemostat. Antigens from spiral and coccoid form were prepared using acid glycine extraction. Enzyme-linked immunosorbent assay (ELISA) was performed to detect serum IgG antibodies against spiral and coccoid forms of H. pylori.

RESULTS: Seroprevalence of H. pylori infection was higher in patients with gastric ulcer (79%) and gastric cancer (83%) than those with non-ulcer dyspepsia (NUD) (44%) and other diseases (45%) (P < 0.05). IgG antibodies against spiral and coccoid antigens were detected in 50.7% (141/278) and 49.6% (138/278), respectively.

CONCLUSION: The spiral and coccoid forms of H. pylori coexist in patients infected with the bacterium.

Keywords: Helicobacter pylori; spiral form; coccoid form; ELISA; helicobacter pylori infection; helicobacter pylori; antigens, bacterial; IgG; antigens, differentiation; antigen-antibody reactions; stomach ulcer; sotamach neoplasms; gastritis