Evaluation of various solutions for small bowel graft preservation

Abstract

AIM: To elucidate the effect of various solutions for small bowel graft preservation in pigs under hypothermic storage.

METHODS: The swine segmental small bowel graft was autotransplanted after it was preserved with lactated Ringer¡äs (LR), Euro-Collins (EC), hyperosmolarity citrate adenine (HC-A) and WMO-1 solu-tions for 10, 18 and 24 h, respectively. The recipient survival rate, morphological structure, graft mucosal energy substances and Na⁺ K⁺ ATPase activity were studied, and graft absorption was estimated with D-xylose absorption test.

RESULTS: The morphological study of the grafts preserved with LR or HC-A solution for 10 hours or with EC and WMO-1 solution for 18 h was nor-mal 6 d after operation. Mucosal ATP, total adenine nucleotides (TAN) contents and Na⁺-K⁺AT-Pase activity of the graft preserved with EC or WMO solution were higher than that of the graft preserved with LR or HC-A solution. Serum level of D-xylose was higher in EC and WMO-1 groups than in LR and HC-A groups when the graft was preserved for 24 h.

CONCLUSIONS: EC and WMO-1 solutions can preserve the swine small bowel up to 18 h, which are superior to LR and HC-A solutions.

Keywords: intestine, small/transplantation; organ preservation; adenosine triphosphate; intestine, small/transplatation; Na(+)-K(+)-exchanging ATPase; adenine nucleotides