Original Articles
Copyright ©The Author(s) 1998. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 15, 1998; 4(1): 7-9
Published online Feb 15, 1998. doi: 10.3748/wjg.v4.i1.7
Tumor radioimmunoimaging of chimeric antibody in nude mice with hepatoma xenograft
Yi Gong, Kang-Da Liu, Ge Zhou, Qiong Xue, Shao-Liang Chen, Zhao-You Tang
Yi Gong, Kang-Da Liu, Ge Zhou, Qiong Xue, Zhao-You Tang, Liver Cancer Institute, Zhongshan Hospital, Shanghai Medical University, Shanghai 200032, China
Shao-Liang Chen, Institute of Nuclear Medicine, Zhongshan Hospital, Shanghai Medical University, Shanghai 200032, China
Yi Gong, female, born in 1971 in Shanghai, graduated from Shanghai Medical University in 1994, Master degree of internal medicine.
Author contributions: All authors contributed equally to the work.
Correspondence to: Prof. Kang-Da Liu, Liver Cancer Institute, Zhongshan Hospital, Shanghai Medical University, Shanghai 200032, China
Telephone: +86-21-64041990 ext. 2295.
Received: September 20, 1997
Revised: November 22, 1997
Accepted: December 20, 1997
Published online: February 15, 1998
Abstract

AIM: To study the radioimmunoimaging (RAII) using the human/mouse chimeric Ab to evaluate its targeting activity in animal models.

METHODS: To chimeric Ab was labeled with 131I. RAII was performed at different intervals after injection of radio-labeled Abs in nude mice with human hepatoma xenograft, and tissue distribution of radioactivity was measured. Comparison was made in the chimeric Ab between the single segment Ab and previous murine mAb against HBxAg.

RESULTS: The experimental objects developed tumor-positive image after 2 days of radio-labeled Abs injection, and the peak accumulation of radioactivity fell on the 7th day. The tumor/liver ratioactivity of the chimeric Ab, single segment Ab, anti-HBx mAb, and the control group was 281 ± 0.21, 2.44 ± 0.16, 4.60 ± 0.19, and 0.96 ± 0.14, respectively.

CONCLUSION: The genetic engineering Abs have a considerable targeting activity which can be used as a novel humanized vector in the targeting treatment of liver cancer.

Keywords: liver neoplasms, experimental; carcinoma, hepatocellular; chimeric antibody; mice, nude; hepatitis B virus; disease models, animal; radioimmunodetection; radioimmunotherapy