Original Research
Copyright ©The Author(s) 1997. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 15, 1997; 3(4): 251-252
Published online Dec 15, 1997. doi: 10.3748/wjg.v3.i4.251
Determination of β-glucuronidase in human colorectal carcinoma cell lines
Shu Feng, Jin-Dan Song
Shu Feng, Jin-Dan Song, Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical University, Shenyang 110001, Liaonin Province, China
Shu Feng, female, born on 1965-02-09 in Hailin, Heilongjiang Province, graduated from Jiamusi Medical Collage in 1986, currently lecturer and postgraduate, having 9 papers published.
Author contributions: All authors contributed equally to the work.
Supported by The National Natural Science Foundation of China, No. 3904005.
Correspondence to: Dr. Shu Feng, Key Laboratory of Cell Biology, Ministry of Public Health of China, China Medical University, Shenyang 110001, Liaonin Province, China
Telephone: +86-24-3916243
Received: March 8, 1997
Revised: May 2, 1997
Accepted: October 28, 1997
Published online: December 15, 1997
Abstract

AIM: To study the relationship between β-glucuronidase and the invasiveness of human colorectal carcinoma cell lines.

METHODS: Six colorectal carcinoma cell lines, including three well-differentiated (CX1, CCL187, and CCL229) and three poorly differentiated ones (CCL227, CCL228, and Clone A), were analyzed by Fischman’s method to determine the concentration of β-glucuronidase in the medium.

RESULTS: Low levels of β-glucuronidase (activity range: 1.29 to 1.96 μg/106 cells·h) were associated with poor invasiveness. This finding was in contrast to the elevated levels of the enzyme (2.46-3.37 μg/106·h) detected in the medium derived from the more aggressively invasive cells (CCL 227, CCL 228, Clone A, and CCL 229).

CONCLUSION: Highly invasive colorectal carcinoma cells secreted higher levels of β-glucuronidase than the poorly invasive cells. Determination of secreted β-glucuronidase might represent a useful in vitro measurement tool to assess the invasiveness of colorectal carcinoma.

Keywords: Colorectal neoplasms; β-glucuronidase invasiveness; Cell lines