Published online Jun 28, 2023. doi: 10.3748/wjg.v29.i24.3793
Peer-review started: April 18, 2023
First decision: April 26, 2023
Revised: May 4, 2023
Accepted: May 22, 2023
Article in press: May 22, 2023
Published online: June 28, 2023
Processing time: 71 Days and 1 Hours
Formyl peptide receptor 2 (Fpr2) is an important receptor in host resistance to bacterial infections. In previous studies, we found that the liver of Fpr2-/- mice is the most severely damaged target organ in bloodstream infections, although the reason for this is unclear.
To investigate the role of Fpr2 in liver homeostasis and host resistance to bacterial infections.
Transcriptome sequencing was performed on the livers of Fpr2-/- and wild-type (WT) mice. Differentially expressed genes (DEGs) were identified in the Fpr2-/- and WT mice, and the biological functions of DEGs were analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) en-richment analysis. Quantitative real time-polymerase chain reaction (qRT-PCR) and western blot (WB) analyses were used to further validate the expression levels of differential genes. Cell counting kit-8 assay was employed to investigate cell survival. The cell cycle detection kit was used to measure the distribution of cell cycles. The Luminex assay was used to analyze cytokine levels in the liver. The serum biochemical indices and the number of neutrophils in the liver were measured, and hepatic histopathological analysis was performed.
Compared with the WT group, 445 DEGs, including 325 upregulated genes and 120 downregulated genes, were identified in the liver of Fpr2-/- mice. The enrichment analysis using GO and KEGG showed that these DEGs were mainly related to cell cycle. The qRT-PCR analysis confirmed that several key genes (CycA, CycB1, Cdc20, Cdc25c, and Cdk1) involved in the cell cycle had significant changes. The WB analysis confirmed a decrease in the expression of CDK1 protein. WRW4 (an antagonist of Fpr2) could inhibit the proliferation of HepG2 cells in a concentration dependent manner, with an increase in the number of cells in the G0/G1 phase, and a decrease in the number of cells in the S phase. Serum alanine aminotransferase levels increased in Fpr2-/- mice. The Luminex assay measurements showed that interleukin (IL)-10 and chemokine (C-X-C motif) ligand (CXCL)-1 levels were significantly reduced in the liver of Fpr2-/- mice. There was no difference in the number of neutrophils, serum C-reactive protein levels, and liver pathology between WT and Fpr2-/- mice.
Fpr2 participates in the regulation of cell cycle and cell proliferation, and affects the expression of IL-10 and CXCL-1, thus playing an important protective role in maintaining liver homeostasis.
Core Tip: Formyl peptide receptor 2 (Fpr2) is an important receptor in host resistance to bacterial infection. After Fpr2 deletion, the pathways involved in the cell cycle are affected, the expression of Cdk1 is downregulated, the proliferation activity of HepG2 cells is reduced, and the distribution of cell cycle is abnormal. After Fpr2 deletion, the permeability of the hepatocyte membrane increases, and the expression of interleukin-10 and Chemokine (C-X-C motif) ligand-1 decreases. These changes reflect the important protective effect of Fpr2 in maintaining liver homeostasis.