Basic Study
Copyright ©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 7, 2017; 23(5): 800-809
Published online Feb 7, 2017. doi: 10.3748/wjg.v23.i5.800
Genomic variability of Helicobacter pylori isolates of gastric regions from two Colombian populations
Andrés Jenuer Matta, Alvaro Jairo Pazos, Javier Andrés Bustamante-Rengifo, Luis Eduardo Bravo
Andrés Jenuer Matta, Luis Eduardo Bravo, Javier Andrés Bustamante, Registro Poblacional de Cáncer de Cali, Department of Pathology, School of Medicine, Universidad del Valle, Cali 760043, Colombia
Alvaro Jairo Pazos, Department of Biology, Universidad de Nariño, Pasto 520002, Colombia
Author contributions: All the authors that were involved in the acquisition and interpretation of the results, read and approved the final manuscript; Matta AJ, Pazos AJ and Bustamante-Rengifo JA conducted the microbiological and molecular tests; Matta AJ and Bravo LE analyzed the data; Matta AJ, Pazos AJ, Bustamante-Rengifo JA and Bravo LE wrote, edited, and revised the manuscript.
Institutional review board statement: All procedures involving human participants were reviewed and approved by the Ethics Committee at Universidad del Valle, Cali, Colombia.
Informed consent statement: All study participants, or their legal guardian, provided informed written consent prior to study enrollment.
Conflict-of-interest statement: The authors declare that there is no conflict of interest related to this study.
Data sharing statement: Technical appendix, statistical code, and dataset available from the corresponding author at andres.matta@correounivalle.edu.co Participants gave informed consent for data sharing.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Andres Jenuer Matta, MSc, PhD, Registro Poblacional de Cáncer de Cali, Department of Pathology, School of Medicine, Universidad del Valle, Street 4B No. 36-00, Building 116, Floor 4, Cali 760043, Colombia. andres.matta@correounivalle.edu.co
Telephone: +57-2-5185623 Fax: +57-2-3212100
Received: July 13, 2016
Peer-review started: July 17, 2016
First decision: August 19, 2016
Revised: September 5, 2016
Accepted: September 14, 2016
Article in press: September 14, 2016
Published online: February 7, 2017
Processing time: 193 Days and 2.5 Hours
Abstract
AIM

To compare the genomic variability and the multiple colonization of Helicobacter pylori (H. pylori) in patients with chronic gastritis from two Colombian populations with contrast in the risk of developing gastric cancer (GC): Túquerres-Nariño (High risk) and Tumaco-Nariño (Low risk).

METHODS

Four hundred and nine patients from both genders with dyspeptic symptoms were studied. Seventy-two patients were included in whom H. pylori was isolated from three anatomic regions of the gastric mucosa, (31/206) of the high risk population of GC (Túquerres) and (41/203) of the low risk population of GC (Tumaco). The isolates were genotyped by PCR-RAPD. Genetic diversity between the isolates was evaluated by conglomerates analysis and multiple correspondence analyses.

RESULTS

The proportion of virulent genotypes of H. pylori was 99% in Túquerres and 94% in Tumaco. The coefficient of similarity of Nei-Li showed greater genetic diversity among isolates of Túquerres (0.13) than those of Tumaco (0.07). After adjusting by age, gender and type of gastritis, the multiple colonization was 1.7 times more frequent in Túquerres than in Tumaco (P = 0.05).

CONCLUSION

In Túquerres, high risk of GC there was a greater probability of multiple colonization by H. pylori. From the analysis of the results of the PCR-RAPD, it was found higher genetic variability in the isolates of H. pylori in the population of high risk for the development of GC.

Keywords: Helicobacter pylori; Pathogenicity islet cag; cagA; vacA; Multiple colonization; PCR-RAPD

Core tip: Multiple colonization of Helicobacter pylori (H. pylori) occurred more frequently in individuals living in the Colombian population with higher risk of gastric cancer (GC) (Túquerres). In the two populations contrasted in relation to the risk of developing GC. (Túquerres high risk and Tumaco low risk) H. pylori was identified with specific genetic characteristics for each region and with varying stages of genomic variability. The diversity of H. pylori dependent of the anatomic regions of the gastric mucosa, obstructs the eradication of the microorganism. Identifying the multiple colonization and evaluating the genetic diversity of H. pylori individuals may be sifted that require particular schemes of early treatment and prevention of the precursor lesions of GC.