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©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
Animal experimental studies using small intestine endoscope
Jin-Hua Liu, Dan-Yang Liu, Li Wang, Li-Ping Han, Zhe-Yu Qi, Hai-Jun Ren, Yan Feng, Feng-Ming Luan, Liang-Tian Mi, Shu-Mei Shan
Jin-Hua Liu, Li Wang, Zhe-Yu Qi, Hai-Jun Ren, Yan Feng, Feng-Ming Luan, Liang-Tian Mi, Department of General Surgery, Affiliated Dalian Municipal Friendship Hospital of Dalian Medical University, Dalian 116001, Liaoning Province, China
Dan-Yang Liu, Department of Endocrinology, Affiliated Dalian Municipal Friendship Hospital of Dalian Medical University, Dalian 116001, Liaoning Province, China
Li-Ping Han, Department of Anesthesiology, Affiliated Dalian Municipal Friendship Hospital of Dalian Medical University, Dalian 116001, Liaoning Province, China
Shu-Mei Shan, Dalian Ming Sheng Technology Development Co., Ltd., Dalian 116001, Liaoning Province, China
Author contributions: Liu JH and Liu DY contributed equally to this paper; Liu JH and Liu DY performed the majority of experiments and analyzed the data; Wang L, Qi ZY and Ren HJ performed the molecular investigations; Han LP was responsible for animal anesthesia; Feng Y and Luan FM treated the animals; Mi LT designed and coordinated the research; Liu JH and Liu DY wrote the paper; Shan SM provided small intestine endoscope.
Supported by Dalian Medical Science Research Project.
Institutional review board statement: This study was approved by the Institutional Review Board of the Affiliated Dalian Municipal Friendship Hospital of Dalian Medical University.
Conflict-of-interest statement: To the best of our knowledge, no conflict of interest exists.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Dan-Yang Liu, PhD, Department of Endocrinology, Affiliated Dalian Municipal Friendship Hospital of Dalian Medical University, 8 Sanba Square, Zhongshan District, Dalian 116001, Liaoning Province, China.
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Received: January 20, 2017
Peer-review started: January 20, 2017
First decision: February 21, 2017
Revised: February 28, 2017
Accepted: March 21, 2017
Article in press: March 21, 2017
Published online: May 28, 2017
Processing time: 126 Days and 8.3 Hours
AIM
To assess the feasibility and safety of a novel enteroscope, negative-pressure suction endoscope in examining the small intestine of a porcine model.
METHODS
In vitro experiments in small intestinal loops from 20 pigs and in vivo experiments in 20 living pigs were conducted.
RESULTS
In in vitro experiments, a negative pressure of > 0.06 MPa was necessary for optimal visualization of the intestine, and this pressure did not cause gross or histological damage to the mucosa. For satisfactory examination of the small intestine in vivo, higher negative pressure (> 1.00 MPa) was required. Despite this higher pressure, the small intestine did not show any gross or microscopic damage in the suctioned areas. The average time of examination in the living animals was 60 ± 7.67 min. The animals did not experience any apparent ill effects from the procedure.
CONCLUSION
Small intestine endoscope was safely performed within a reasonable time period and enabled complete visualization of the intestine in most cases.
Core tip: The main component of endoscopes is an ultrafine tubular endoscope, with an added external propeller, to assist in the migration of the endoscope through the intestine.
