Zhou HB, Chen JM, Shao LM, Chen ZG. Apoptosis of human pancreatic carcinoma cell-1 cells induced by Yin Chen Hao Decoction. World J Gastroenterol 2015; 21(27): 8352-8357 [PMID: 26217086 DOI: 10.3748/wjg.v21.i27.8352]
Corresponding Author of This Article
Hai-Bo Zhou, MD, Department of Gastroenterology, the Second Affiliated Hospital of Zhejiang University School of Medicine, No. 88, Jiefang Road, Hangzhou 310009, Zhejiang Province, China. zhouhaibohz@163.com
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Hai-Bo Zhou, Jing-Ming Chen, Li-Ming Shao, Zhi-Gang Chen, Department of Gastroenterology, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, Zhejiang Province, China
Author contributions: Zhou HB designed and coordinated the research and wrote the paper; Chen JM and Chen ZG performed the majority of experiments; and Shao LM analyzed the data.
Supported by Traditional Chinese Medicine Administration of Zhejiang Province, China, No. 2014ZA068.
Institutional review board statement: The study was reviewed and approved by Chinese Medicine Administration Bureau of Zhejiang.
Conflict-of-interest statement: The authors declare no conflict of interests.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Hai-Bo Zhou, MD, Department of Gastroenterology, the Second Affiliated Hospital of Zhejiang University School of Medicine, No. 88, Jiefang Road, Hangzhou 310009, Zhejiang Province, China. zhouhaibohz@163.com
Telephone: +86-571-87783564
Received: January 24, 2015 Peer-review started: January 25, 2015 First decision: February 10, 2015 Revised: March 9, 2015 Accepted: April 17, 2015 Article in press: April 17, 2015 Published online: July 21, 2015 Processing time: 178 Days and 19.7 Hours
Abstract
AIM: To evaluate human pancreatic carcinoma cell line (PANC-1) cells apoptosis and Bcl-2 and Bax expression induced by Yin Chen Hao Decoction (YCHD).
METHODS: The cell growth inhibitory rate was determined by MTT assay. Apoptosis of PANC-1 cells before and after treatment with YCHD was determined by TUNEL staining. Expression of the apoptosis-associated genes, Bcl-2 and Bax, was detected by immunohistochemical staining and reverse transcription -PCR.
RESULTS: YCHD inhibited the growth of PANC-1 cells. Following treatment with YCHD for 24-96 h, the apoptotic rate of PANC-1 cells increased with time. In addition, the positive rate of Bcl-2 protein expression decreased in a time-dependent manner, whereas the positive rate of Bax protein expression increased in a time-dependent manner. Following treatment of with YCHD for 24-96h, expression of BAX mRNA increased gradually and BCL-2 mRNA reduced gradually with time.
CONCLUSION: YCHD induces apoptosis of PANC-1 cells mediated in part via up-regulation of BAX and down-regulation of BCL-2.
Core tip: Yin Chen Hao Decoction (YCHD) inhibits the growth of pancreatic carcinoma cells. Following treatment with YCHD for 24-96 h, the apoptotic rates in these cells is increased by YCHD, which is accompanied by time-dependent increased expression of Bax and decreased expression of Bcl-2. YCHD-induced apoptosis of these cells may be mediated by upregulation of BAX and downregulation of BCL-2.
