Brief Article
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World J Gastroenterol. Mar 7, 2014; 20(9): 2397-2402
Published online Mar 7, 2014. doi: 10.3748/wjg.v20.i9.2397
RAGE gene three polymorphisms with Crohn's disease susceptibility in Chinese Han population
Zheng-Ting Wang, Jia-Jia Hu, Rong Fan, Jie Zhou, Jie Zhong
Zheng-Ting Wang, Rong Fan, Jie Zhou, Jie Zhong, Department of Gastroenterology, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China
Jia-Jia Hu, Department of Nuclear Medicine, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200025, China
Author contributions: Wang ZT and Hu JJ contributed equally to this work; Zhong J contributed to the conception and design of the research; Zhong J corrected and revised the paper; Fan R and Zhou J collected samples; Wang ZT and Hu JJ performed the research and drafted the paper.
Correspondence to: Jie Zhong, Professor, Department of Gastroenterology, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, 600 Yushan Road, Shanghai 200025, China. jimmyzj64@medmail.com.cn
Telephone: +86-21-64370045 Fax: +86-21-64370045
Received: July 7, 2013
Revised: October 18, 2013
Accepted: December 12, 2013
Published online: March 7, 2014
Processing time: 242 Days and 3.5 Hours
Abstract

AIM: To investigate the association of three polymorphisms in the receptor for advanced glycation end product (RAGE) gene with Crohn’s disease (CD) risk in a Chinese population.

METHODS: A hospital-based case-control association study involving 312 CD patients and 479 healthy controls was conducted. Peripheral blood samples were collected from 791 study subjects, and genomic DNA was extracted. Genotyping was performed using polymerase chain reaction-ligase detection reaction method. The association between polymorphic genotype and CD predisposition was determined using odds ratio and 95% confidence interval (CI). Data were analyzed using Haplo.stats program.

RESULTS: Significant differences were observed between patients and controls in allele/genotype distributions of rs1800624 (Pallele=0.012; Pgenotype=0.005) and in allele distributions of rs2070600 (P=0.02). The risk for CD associated with the rs1800624-A mutant allele decreased by 36% (95%CI: 0.47-0.88, P = 0.005) under the additive model and by 35% (95%CI: 0.46-0.91, P=0.013) under the dominant model. Carriers of rs2070600-A mutant allele showed a 37% (95%CI: 1.02-1.83, P=0.036) increased risk of developing CD relative to the GG genotype carriers. In haplotype analysis, haplotype T-A-G (in the order rs1800625, rs1800624, and rs2070600) decreased the odds of CD by 33% (95%CI: 0.49-0.94, P=0.018).

CONCLUSION: CD is an immune-related disease with genetic predisposition. Genetic defects in the RAGE gene are strongly associated with CD in Chinese population.

Keywords: Receptor for advanced glycation end product; Polymorphism; Crohn’s diseases; Susceptibility; Association study

Core tip: The receptor for advanced glycation end products (RAGE) is a pattern recognition receptor involved in several pathophysiological processes associated with inflammation. Therefore, we considered that RAGE gene is a candidate gene susceptible to Crohn’s disease (CD). This study is the first to investigate the association of the three most commonly studied polymorphisms in RAGE gene with CD risk in a Chinese population. The results suggest that RAGE rs1800624 and rs2070600 polymorphisms are associated with CD occurrence. The present findings support the hypothesis that a genetically impaired innate defense immunity system is a predisposing factor in the etiology of CD.