Review
Copyright ©2014 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Jan 14, 2014; 20(2): 475-485
Published online Jan 14, 2014. doi: 10.3748/wjg.v20.i2.475
Limitations of liver biopsy and non-invasive diagnostic tests for the diagnosis of nonalcoholic fatty liver disease/nonalcoholic steatohepatitis
Yoshio Sumida, Atsushi Nakajima, Yoshito Itoh
Yoshio Sumida, Yoshito Itoh, Department of Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan
Atsushi Nakajima, Division of Gastroenterology, Yokohama City University Graduate School of Medicine, Yokohama 236-0004, Japan
Author contributions: Nakajima A and Ioh Y equally contributed to this work; Sumida Y wrote the manuscript.
Supported by Scholarship Funds from MSD Co., Ltd. (to Sumida Y); Scholarship Funds from MSD Co., Ltd., and Dainippon Sumitomo Pharma Co., Ltd. (to Ioh Y)
Correspondence to: Yoshio Sumida, MD, PhD, Department of Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine, Kamigyo-ku, Kyoto 602-8566, Japan. sumida@koto.kpu-m.ac.jp
Telephone: +81-75-2515519 Fax: +81-75-2510710
Received: July 31, 2013
Revised: September 29, 2013
Accepted: November 3, 2013
Published online: January 14, 2014
Processing time: 171 Days and 23.2 Hours
Abstract

It is estimated that 30% of the adult population in Japan is affected by nonalcoholic fatty liver disease (NAFLD). Fatty changes of the liver are generally diagnosed using imaging methods such as abdominal ultrasonography (US) and computed tomography (CT), but the sensitivity of these imaging techniques is low in cases of mild steatosis. Alanine aminotransferase levels may be normal in some of these patients, warranting the necessity to establish a set of parameters useful for detecting NAFLD, and the more severe form of the disease, nonalcoholic steatohepatitis (NASH). Although liver biopsy is currently the gold standard for diagnosing progressive NASH, it has many drawbacks, such as sampling error, cost, and risk of complications. Furthermore, it is not realistic to perform liver biopsies on all NAFLD patients. Diagnosis of NASH using various biomarkers, scoring systems and imaging methods, such as elastography, has recently been attempted. The NAFIC score, calculated from the levels of ferritin, fasting insulin, and type IV collagen 7S, is useful for the diagnosis of NASH, while the NAFLD fibrosis score and the FIB-4 index are useful for excluding NASH in cases of advanced fibrosis. This article reviews the limitations and merits of liver biopsy and noninvasive diagnostic tests in the diagnosis of NAFLD/NASH.

Keywords: Nonalcoholic fatty liver disease; Liver biopsy; Steatosis; Fibrosis; Nonalcoholic steatohepatitis

Core tip: Liver biopsies remain a gold standard, although the procedure has several limitations for the diagnosis of nonalcoholic steatohepatitis (NASH). The NAFIC score, calculated from the levels of ferritin, fasting insulin and type IV collagen 7S, is useful for diagnosing NASH, while the nonalcoholic fatty liver disease fibrosis score and the FIB-4 index are useful for excluding NASH in cases of advanced fibrosis.