Published online Sep 15, 1996. doi: 10.3748/wjg.v2.iSuppl1.166
Revised: January 21, 1996
Accepted: June 15, 1996
Published online: September 15, 1996
AIM: Various methods for measurement of gastric emptying have received widely study. In clinic, 99mTc-sulphur colloid (99mTc-Sc), 131I fiber and 111In-DAPA have been accepted as a valuable markers for digestive solid, non-digestive solid and liquid component respectively. However, half life period for 111In is only 1.5 h which is so short that incur a problem for shipment, and non digestive marker of 131I fiber cannot response for the physiological function of the gut. In this study, we investigated the validity of 131I-bovine serum album (131I-BSA), a possible candidate for digestive solid marker, together with 99mTc-DTPA as liquid marker in the determination of gastric emptying for solid and liquid simultaneously.
METHODS: In vitro, the solid phase of 131I-BSA labeled EGG were incubated in human gastric juice (pH 2.0), 1 N NaCl (pH 7.0) and 0.1 N HCl (pH 1.0) over a period of 2 h for analysis of its stability. Stability for the liquid phase of 99mTc-DTPA and another solid phase of 99mTc-Sc were also studied in the same manner. In vivo, gastric emptying were measured using 131I-BSA as solid marker and 99mTc-DTPA as liquid marker in 35 patients with chronic gastric (group CG) and 10 healthy controls (group HC) to explore the ability of a combination of these 2 labels in the distinguishment of solid and liquid emptying.
RESULTS: Gastric emptying patterns of solid and liquid showed significant difference: solid emptying showed an evidence of lag phase and a significant prolongation of half emptying time for solid than that for liquid. Comparing to group HC, group CG showed a prolongation of solid lag time in 14 patients, slower half solid emptying in 15 patients and retarded liquid emptying rate in 12 patients. Solid emptying parameters for 131I-BSA were significant correlated to those for 99mTc-Sc (r = 0.989, P < 0.01). Variability of solid emptying for 131I-BSA and 99mTc-Sc, showed a maximal difference of 9.2% and a minimal difference of 0.8%.
CONCLUSION: 131I-BSA labeled EGG showed a higher stability in different solutions and had a pattern of emptying similar to that of 99mTc-Sc, suggesting that 131I-BSA is a valuable digestive solid marker for detection of solid emptying. A combination of use of 131I-BSA, 99mTc-Sc, and 111In-EHIDA imultaneously in the measurement of gastric emptying and bile reflux is anticipated.