Wang LD, Zhou Q, Gao SS, Li YX, Yang WC. Measurements of cell proliferation in esophageal and gastric cardia epithelia of subjects in a high incidence area for esophageal cancer. World J Gastroenterol 1996; 2(2): 82-85 [DOI: 10.3748/wjg.v2.i2.82]
Corresponding Author of This Article
Dr. Li-Dong Wang, Professor, Director of Laboratory for Cancer Research, Henan Medical University, Zhengzhou 450052, Henan Province, China
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Clinical Articles
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World J Gastroenterol. Jun 25, 1996; 2(2): 82-85 Published online Jun 25, 1996. doi: 10.3748/wjg.v2.i2.82
Measurements of cell proliferation in esophageal and gastric cardia epithelia of subjects in a high incidence area for esophageal cancer
Li-Dong Wang, Qi Zhou, Shan-Shan Gao, Yong-Xin Li, Wan-Cai Yang
Li-Dong Wang, Professor of Oncology and Pathology of Henan Medical University, China; adjunct Professor of Rutgers University, the State University of New Jersey, United States; tutor for MD degree students; active member of American Association for Cancer Research; life member of the Society of Chinese Bioscientists in America; having 65 papers and 2 books published, and 20 papers presented in international cancer research meetings in the United States and Canada; research fellow at the Memorial Sloan-Kettering Cancer Research Center in New York from 1987-1988; postdoctoral training at Rutgers University in New Jersey from 1991-1994; visiting scholar at Rutgers University in New Jersey, May-August 1995 and June-September 1996; having received award for cancer research by Upjohn, Bristol-Myers and Cytogen in the United States; enrolled in the “Dictionary of Modern Chinese Scientists and Inventors”; Director of Laboratory for Cancer Research, Henan Medical University, Zhengzhou 450052, Henan Province, China
Qi Zhou, Shan-Shan Gao, Yong-Xin Li, Wan-Cai Yang, Henan Medical University, Zhengzhou 450052, Henan Province, China
Author contributions: All authors contributed equally to the work.
Supported by The Foundation of Chinese Education Commission and Scientific Foundation for Excellent Young Scientists in Henan.
Correspondence to: Dr. Li-Dong Wang, Professor, Director of Laboratory for Cancer Research, Henan Medical University, Zhengzhou 450052, Henan Province, China
Telephone: +86-371-6970165
Received: April 29, 1996 Revised: May 25, 1996 Accepted: June 10, 1996 Published online: June 25, 1996
Abstract
AIM: To determine the proliferation patterns of normal and different precancerous lesions of esophageal and gastric cardia epithelia by measuring levels of proliferating cell nuclear antigen (PCNA), Ki-67 and bromodeoxyuridine (BudR) incorporation.
METHODS: One-hundred-and-seventy-five esophageal biopsies and 45 gastric cardia biopsies were collected from symptom-free subjects in Huixian. Of these, 24 esophageal biopsies were incubated with BudR. The avidin-biotin-peroxidase complex (ABC) method was used to detect PCNA, Ki-67 and BudR. Quantitative data of the immunostaining results were recorded as the number of positive cells per mm2 of the biopsied epithelium.
RESULTS: Intense immunostaining for PCNA, Ki-67 and BudR was observed in the cell nuclei of normal tissues and of tissues with different severities of precancerous lesions. For esophageal biopsies, the numbers of both PCNA and Ki-67 increased significantly as the epithelia progressed from normal to basal cell hyperplasia (BCH) and to dysplasia (DYS). The number of PCNA- and Ki-67-positive cells was three times higher than that of BudR in the same category of BCH. For cardia biopsies, the number of Ki-67-positive cells was lower in normal tissues and increased significantly from chronic superficial gastritis (CSG) to chronic atrophic gastritis (CAG) and to DYS.
CONCLUSION: The staining patterns for PCNA and Ki-67 were correlated with the histopathology of the esophagus and gastric cardia. These methods may be useful for screening subjects at high risk for esophageal and gastric cardia cancers and for monitoring the effect of chemoprevention. PCNA is relatively easy to analyze and may prove to be very useful in studies on esophageal cancer.
