Fang XF, Hou ZB, Dai XZ, Chen C, Ge J, Shen H, Li XF, Yu LK, Yuan Y. Special AT-rich sequence-binding protein 1 promotes cell growth and metastasis in colorectal cancer. World J Gastroenterol 2013; 19(15): 2331-2339 [PMID: 23613626 DOI: 10.3748/wjg.v19.i15.2331]
Corresponding Author of This Article
Dr. Ying Yuan, Department of Medical Oncology, Second Affiliated Hospital, Zhejiang University College of Medicine, 88 Jiefang Road, Hangzhou 310000, Zhejiang Province, China. yuanying1999@zju.edu.cn
Research Domain of This Article
Oncology
Article-Type of This Article
Original Article
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Xue-Feng Fang, Hong Shen, Ying Yuan, Xiao-Fang Li, Department of Medical Oncology, Second Affiliated Hospital, Zhejiang University College of Medicine, Hangzhou 310000, Zhejiang Province, China
Zhi-Bo Hou, Li-Ke Yu, First Department of Respiratory Medicine, Nanjing Chest Hospital, Nanjing 210029, Jiangsu Province, China
Xin-Zheng Dai, Liver Transplantation Center, First Affiliated Hospital of Nanjing Medical University, Key Laboratory of Living Donor Liver Transplantation, Ministry of Public Health, Nanjing 210029, Jiangsu Province, China
Cong Chen, Department of Gynecology of Traditional Chinese Medicine, Jiangsu Provincial Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Traditional Chinese Medicine, Nanjing 210029, Jiangsu Province, China
Jing Ge, Department of Endocrinology, Jiangsu Provincial Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Traditional Chinese Medicine, Nanjing 210029, Jiangsu Province, China
Author contributions: Fang XF, Hou ZB and Dai XZ contributed equally to this work; Hou ZB, Dai XZ and Chen C carried out the molecular genetic studies; Chen C, Ge J and Li XF participated in the animal study; Hou ZB, Chen C and Ge J analyzed final data; Fang XF, Hou ZB and Shen H drafted the manuscript; Yuan Y designed this research; all authors read and approved the final manuscript.
Supported by The National Natural Science Foundation of China, No. 81101580
Correspondence to: Dr. Ying Yuan, Department of Medical Oncology, Second Affiliated Hospital, Zhejiang University College of Medicine, 88 Jiefang Road, Hangzhou 310000, Zhejiang Province, China. yuanying1999@zju.edu.cn
Telephone: +86-571-87784795 Fax: +86-571-87767088
Received: December 2, 2012 Revised: January 13, 2013 Accepted: February 2, 2013 Published online: April 21, 2013 Processing time: 138 Days and 19.1 Hours
Abstract
AIM: To evaluate the expression of special AT-rich sequence-binding protein 1 (SATB1) gene in colorectal cancer and its role in colorectal cancer cell proliferation and invasion.
METHODS: Immunohistochemistry was used to detect the protein expression of SATB1 in 30 colorectal cancer (CRC) tissue samples and pair-matched adjacent non-tumor samples. Cell growth was investigated after enhancing expression of SATB1. Wound-healing assay and Transwell assay were used to investigate the impact of SATB1 on migratory and invasive abilities of SW480 cells in vitro. Nude mice that received subcutaneous implantation or lateral tail vein were used to study the effects of SATB1 on tumor growth or metastasis in vivo.
RESULTS: SATB1 was over-expressed in CRC tissues and CRC cell lines. SATB1 promotes cell proliferation and cell cycle progression in CRC SW480 cells. SATB1 overexpression could promote cell growth in vivo. In addition, SATB1 could significantly raise the ability of cell migration and invasion in vitro and promote the ability of tumor metastasis in vivo. SATB1 could up-regulate matrix metalloproteases 2, 9, cyclin D1 and vimentin, meanwhile SATB1 could down-regulate E-cadherin in CRC.
CONCLUSION: SATB1 acts as a potential growth and metastasis promoter in CRC. SATB1 may be useful as a therapeutic target for CRC.
