Chen YH, Lan T, Li J, Qiu CH, Wu T, Gou HJ, Lu MQ. Gardenia jasminoides attenuates hepatocellular injury and fibrosis in bile duct-ligated rats and human hepatic stellate cells. World J Gastroenterol 2012; 18(48): 7158-7165 [PMID: 23326120 DOI: 10.3748/wjg.v18.i48.7158]
Corresponding Author of This Article
Min-Qiang Lu, MD, Professor, Department of Hepatology, The Third Affiliated Hospital of Sun Yat-sen University, No. 600 Tianhe Road, Guangzhou 510630, Guangdong Province, China. lantiansci@yahoo.com
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World J Gastroenterol. Dec 28, 2012; 18(48): 7158-7165 Published online Dec 28, 2012. doi: 10.3748/wjg.v18.i48.7158
Gardenia jasminoides attenuates hepatocellular injury and fibrosis in bile duct-ligated rats and human hepatic stellate cells
Ying-Hua Chen, Tian Lan, Jing Li, Chun-Hui Qiu, Teng Wu, Hong-Ju Gou, Min-Qiang Lu
Ying-Hua Chen, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China
Tian Lan, Teng Wu, Hong-Ju Gou, Institute of Vascular Biology, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong Province, China
Jing Li, Guangzhou PTDS Biotechnology Science and Technology Co. Ltd., Guangzhou 510540, Guangdong Province, China
Chun-Hui Qiu, Min-Qiang Lu, Department of Hepatology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, Guangdong Province, China
Author contributions: Chen YH and Lan T designed and performed the study, collected and analyzed the data, wrote and revised the manuscript, and contributed equally to this work; Li J and Qiu CH contributed to the acquisition of data; Wu T and Gou HJ participated in the study design and experiments; Lu MQ contributed to the conception and design of the study as well as funding application; and all authors have approved the final version to be published.
Supported by The Natural Science Foundation of China, No. 81170450 to Lu MQ and No. 81200308 to Lan T; The PhD Start-up Fund of Natural Science Foundation of Guangdong Province, China, No. S2012040008026; and The New Star of Science and Technology Foundation of Zhu Jiang in Guangzhou City
Correspondence to: Min-Qiang Lu, MD, Professor, Department of Hepatology, The Third Affiliated Hospital of Sun Yat-sen University, No. 600 Tianhe Road, Guangzhou 510630, Guangdong Province, China. lantiansci@yahoo.com
Telephone: +86-20-87333774 Fax: +86-20-87331220
Received: June 19, 2012 Revised: October 10, 2012 Accepted: October 16, 2012 Published online: December 28, 2012
Abstract
AIM: To investigate the anti-hepatofibrotic effects of Gardenia jasminoides in liver fibrosis.
METHODS: Male Sprague-Dawley rats underwent common bile duct ligation (BDL) for 14 d and were treated with Gardenia jasminoides by gavage. The effects of Gardenia jasminoides on liver fibrosis and the detailed molecular mechanisms were also assessed in human hepatic stellate cells (LX-2) in vitro.
RESULTS: Treatment with Gardenia jasminoides decreased serum alanine aminotransferase (BDL vs BDL + 100 mg/kg Gardenia jasminoides, 146.6 ± 15 U/L vs 77 ± 6.5 U/L, P = 0.0007) and aspartate aminotransferase (BDL vs BDL + 100 mg/kg Gardenia jasminoides, 188 ± 35.2 U/L vs 128 ± 19 U/L, P = 0.005) as well as hydroxyproline (BDL vs BDL + 100 mg/kg Gardenia jasminoides, 438 ± 40.2 μg/g vs 228 ± 10.3 μg/g liver tissue, P = 0.004) after BDL. Furthermore, Gardenia jasminoides significantly reduced liver mRNA and/or protein expression of transforming growth factor β1 (TGF-β1), collagen type I (Col I) and α-smooth muscle actin (α-SMA). Gardenia jasminoides significantly suppressed the upregulation of TGF-β1, Col I and α-SMA in LX-2 exposed to recombinant TGF-β1. Moreover, Gardenia jasminoides inhibited TGF-β1-induced Smad2 phosphorylation in LX-2 cells.
CONCLUSION: Gardenia jasminoides exerts antifibrotic effects in the liver fibrosis and may represent a novel antifibrotic agent.
