Hezova R, Kovarikova A, Bienertova-Vasku J, Sachlova M, Redova M, Vasku A, Svoboda M, Radova L, Kiss I, Vyzula R, Slaby O. Evaluation of SNPs in miR-196-a2, miR-27a and miR-146a as risk factors of colorectal cancer. World J Gastroenterol 2012; 18(22): 2827-2831 [PMID: 22719192 DOI: 10.3748/wjg.v18.i22.2827]
Corresponding Author of This Article
Ondrej Slaby, PhD, Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Zluty kopec 7, 656 53 Brno, Czech. slaby@mou.cz
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Brief Article
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World J Gastroenterol. Jun 14, 2012; 18(22): 2827-2831 Published online Jun 14, 2012. doi: 10.3748/wjg.v18.i22.2827
Evaluation of SNPs in miR-196-a2, miR-27a and miR-146a as risk factors of colorectal cancer
Renata Hezova, Alena Kovarikova, Julie Bienertova-Vasku, Milana Sachlova, Martina Redova, Anna Vasku, Marek Svoboda, Lenka Radova, Igor Kiss, Rostislav Vyzula, Ondrej Slaby
Renata Hezova, Alena Kovarikova, Milana Sachlova, Martina Redova, Marek Svoboda, Igor Kiss, Rostislav Vyzula, Ondrej Slaby, Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Zluty kopec 7, 656 53 Brno, Czech
Julie Bienertova-Vasku, Anna Vasku, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Kamenice 5, 625 00 Brno, Czech
Martina Redova, Ondrej Slaby, Central European Institute of Technology, Masaryk University, 625 00 Brno, Czech
Lenka Radova, Laboratory of Experimental Medicine, Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University and Palacky University Affiliated Hospital Olomouc, 775 15 Olomouc, Czech
Author contributions: Hezova R and Kovarikova A performed DNA purifications and single nucleotide polymorphism analysis; Sachlova M, Vasku A, Svoboda M, Kiss I and Vyzula R collected DNA samples and clinical data from patients and controls involved in the study; Radova L performed statistical evaluation of the collected data; Bienertova-Vasku J, Redova M and Slaby O participated in manuscript preparation; and Slaby O designed the study, performed the data analysis and interpretation, and critically revised the manuscript.
Supported by Czech Ministry of Health, No. NS 10352-3/2009, NS/9814 and project No. MZ0MOU2005; the Ministry of Education, Youth and Sports for the project BBMRI CZ, No. LM2010004; and the project ‘‘CEITEC-Central European Institute of Technology’’, No. CZ.1.05/1.1.00/02.0068
Correspondence to: Ondrej Slaby, PhD, Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Zluty kopec 7, 656 53 Brno, Czech. slaby@mou.cz
Telephone: +420-5-43136902 Fax: +420-5-43136902
Received: August 13, 2011 Revised: September 24, 2011 Accepted: April 12, 2012 Published online: June 14, 2012
Abstract
AIM: To investigate whether selected single nucleotide polymorphisms (SNPs) in miR-196a2, miR-27a and miR-146a genes are associated with sporadic colorectal cancer (CRC).
METHODS: In order to investigate the effect of these SNPs in CRC, we performed a case-control study of 197 cases of sporadic CRC and 212 cancer-free controls originating from the Central-European Caucasian population using TaqMan Real-Time polymerase chain reaction and allelic discrimination analysis.
RESULTS: The genotype and allele frequencies of SNPs were compared between the cases and the controls. None of the performed analysis showed any statistically significant results.
CONCLUSION: Our data suggest a lack of association between rs11614913, rs895819 and rs2910164 and colorectal cancer risk in the Central-European Caucasian population, a population with an extremely high incidence of sporadic colorectal cancer.
