Brief Article
Copyright ©2011 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Apr 14, 2011; 17(14): 1895-1902
Published online Apr 14, 2011. doi: 10.3748/wjg.v17.i14.1895
Down-regulation of miR-622 in gastric cancer promotes cellular invasion and tumor metastasis by targeting ING1 gene
Xiao-Bo Guo, Chang-Qing Jing, Le-Ping Li, Li Zhang, Yu-Long Shi, Jin-Shen Wang, Jing-Lei Liu, Chen-Sheng Li
Xiao-Bo Guo, Chang-Qing Jing, Le-Ping Li, Li Zhang, Yu-Long Shi, Jin-Shen Wang, Jing-Lei Liu, Chen-Sheng Li, Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwu Weiqi Road, Jinan 250021, Shandong Province, China
Author contributions: Guo XB and Jing CQ contributed equally to this work; Li LP designed the research; Zhang L and Shi YL prepared gastric cancer tissues; Wang JS and Liu JL analyzed quantitative real-time PCR; Li CS, Guo XB and Jing CQ analyzed the data and wrote the manuscript.
Supported by Grants from Science Foundation of Shandong Province of China (2003-23), Key Research Project from Shandong Science and Technology Commission, No. 2005GG3202066
Correspondence to: Le-Ping Li, Professor, Department of Gastrointestinal Surgery, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwu Weiqi Road, Jinan 250021, Shandong Province, China. lileping@medmail.com.cn
Telephone: +86-531-88227222 Fax: +86-531-88496966
Received: November 23, 2010
Revised: January 18, 2011
Accepted: January 25, 2011
Published online: April 14, 2011
Abstract

AIM: To evaluate the biological and clinical characteristics of miR-622 in gastric cancer.

METHODS: We analyzed the expression of miR-622 in 57 pair matched gastric neoplastic and adjacent non-neoplastic tissues by quantitative real-time polymerase chain reaction. Functional analysis of miR-622 expression was assessed in vitro in gastric cancer cell lines with miR-622 precursor and inhibitor. The roles of miR-622 in tumorigenesis and tumor metastasis were analyzed using a stable miR-622 expression plasmid in nude mice. A luciferase reporter assay was used to assess the effect of miR-622 on inhibitor of growth family, member 1 (ING1) expression.

RESULTS: Expression of miR-622 was down-regulated in gastric cancer. MiR-622 was found involved in differentiation and lymphatic metastasis in human gastric cancer. Ectopic expression of miR-622 promoted invasion, tumorigenesis and metastasis of gastric cancer cells both in vitro and in vivo. ING1 is a direct target of miR-622.

CONCLUSION: These findings help clarify the molecular mechanisms involved in gastric cancer metastasis and indicate that miR-622 modulation may be a bona fide treatment of gastric cancer.

Keywords: MicroRNA; MiR-622; Gastric cancer; Metastasis; Inhibitor of growth family member 1