Sfar I, Aleya WB, Mouelhi L, Aouadi H, Rhomdhane TB, Makhlouf M, Ayed-Jendoubi S, Gargaoui H, Najjar T, Abdallah TB, Ayed K, Gorgi Y. Lymphoid tyrosine phosphatase R620W variant and inflammatory bowel disease in Tunisia. World J Gastroenterol 2010; 16(4): 479-483 [PMID: 20101775 DOI: 10.3748/wjg.v16.i4.479]
Corresponding Author of This Article
Dr. Ben Aleya Walid, Laboratory of Immunology, Charles Nicolle Hospital, Boulevard 9 Avril, Tunis 1006, Tunisia. b_a_w@hotmail.fr
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Brief Article
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World J Gastroenterol. Jan 28, 2010; 16(4): 479-483 Published online Jan 28, 2010. doi: 10.3748/wjg.v16.i4.479
Lymphoid tyrosine phosphatase R620W variant and inflammatory bowel disease in Tunisia
Imen Sfar, Walid Ben Aleya, Leila Mouelhi, Houda Aouadi, Thouraya Ben Rhomdhane, Mouna Makhlouf, Salwa Ayed-Jendoubi, Houda Gargaoui, Taoufik Najjar, Taieb Ben Abdallah, Khaled Ayed, Yousr Gorgi
Imen Sfar, Walid Ben Aleya, Leila Mouelhi, Houda Aouadi, Thouraya Ben Rhomdhane, Mouna Makhlouf, Salwa Ayed-Jendoubi, Houda Gargaoui, Taoufik Najjar, Taieb Ben Abdallah, Khaled Ayed, Yousr Gorgi, Laboratory of Immunology, Charles Nicolle Hospital, Tunis 1006, Tunisia
Mouelhi Leila, Najjar Taoufik, Department of Gastroenterology, Charles Nicolle Hospital, Tunis 1006, Tunisia
Author contributions: Ben Aleya W, Sfar I, Aouadi H, Makhlouf M, Ben Rhomdhane T and Ayed-Jendoubi S performed the majority of the experiments; Mouelhi L and Najjar T collected all the human material; Ben Abdallah T, Ayed K and Gorgi Y designed, supervised and provided financial support for this work; and Ben Aleya W wrote the manuscript.
Supported by Laboratory of Immunology, Charles Nicolle Hospital
Correspondence to: Dr. Ben Aleya Walid, Laboratory of Immunology, Charles Nicolle Hospital, Boulevard 9 Avril, Tunis 1006, Tunisia. b_a_w@hotmail.fr
Telephone: +216-98-478866 Fax: +216-71-561156
Received: August 12, 2009 Revised: September 23, 2009 Accepted: September 30, 2009 Published online: January 28, 2010
Abstract
AIM: To assess the possible association between PTPN22 (R620W) gene polymorphism and inflammatory bowel disease (IBD).
METHODS: One hundred and sixty-four patients with IBD [105 Crohn’s disease (CD) and 59 ulcerative colitis (UC)] and 100 healthy controls were recruited. Genotyping of the PTPN22 gene 1858C→T polymorphism was performed by restriction fragment length polymorphism-polymerase chain reaction with RsaI digestion.
RESULTS: The genotypic and allelic frequencies of (R620W) PTPN22 gene polymorphism reveal a significant association of the PTPN22 620-W allele with IBD, compared to the healthy control group (OR: 17.81, 95% CI: 4.18-21.86, P = 0.00001). Nevertheless, no difference in this polymorphism was found between CD and UC patients. No significant association was found between the frequencies of genotypes of the PTPN22 gene with either the clinical features such as sex, age, age at disease onset, and extent of colitis, or the production of serological markers (anti-Saccharomyces cerevisiae antibody in CD and perinuclear anti-neutrophil cytoplasmic antibody in UC).
CONCLUSION: These observations confirm the association of IBD susceptibility with the PTPN22 1858T (620-W) allele in Tunisian patients.
