Original Article
Copyright ©2010 Baishideng. All rights reserved.
World J Gastroenterol. Jan 28, 2010; 16(4): 445-452
Published online Jan 28, 2010. doi: 10.3748/wjg.v16.i4.445
Two stomach-originated lactobacillus strains improve Helicobacter pylori infected murine gastritis
Yi Cui, Chun-Lian Wang, Xiao-Wei Liu, Xue-Hong Wang, Lin-Lin Chen, Xin Zhao, Nian Fu, Fang-Gen Lu
Yi Cui, Chun-Lian Wang, Xiao-Wei Liu, Xue-Hong Wang, Xin Zhao, Nian Fu, Fang-Gen Lu, Department of Gastroenterology, The Second Xiangya Hospital of Central South University, Changsha 410011, Hunan Province, China
Lin-Lin Chen, Department of Gastroenterology, Xiangya Hospital of Central South University, Changsha 410008, Hunan Province, China
Author contributions: Cui Y and Wang CL contributed equally to this work; Cui Y, Wang CL, Liu XW and Lu FG designed the research; Cui Y, Wang CL, Wang XH, Chen LL and Zhao X performed the research; Chen LL, Zhao X and Fu N analyzed and interpreted the data; Cui Y, Wang CL, Liu XW and Lu FG wrote the paper.
Correspondence to: Fang-Gen Lu, Professor, Department of Gastroenterology, The Second Xiangya Hospital of Central South University, Changsha 410011, Hunan Province, China. lufanggenyao@163.com
Telephone: +86-731-85295888 Fax: +86-731-84807725
Received: November 12, 2009
Revised: December 3, 2009
Accepted: December 10, 2009
Published online: January 28, 2010
Abstract

AIM: To investigate the potential anti-Helicobacter pylori (H. pylori) and anti-inflammation in vivo effects of two lactobacillus strains from human stomach.

METHODS: Forty H. pylori infected Balb/c mice were randomly divided into 4 groups: proton pump inhibitor and antibiotics triple treated group, Lactobacillus fermenti (L. fermenti) treated group, Lactobacillus acidophilus treated group and normal saline control group. Ten uninfected mice were also included as blank control group. The infection of H. pylori was detected by rapid urease tests, Giemsa staining and bacterial culture. The colonization of H. pylori was assessed in bacterial density score and gastric inflammation was assessed in histological score. The colonization of L. fermenti was performed by fluorescent probe.

RESULTS: Histopathologic evaluation showed significant release of mucosal inflammation in gastric antrum and gastric body in lactobacillus treated groups and triple treated group. H. pylori eradication rate in both lactobacillus treated groups and triple treated group were higher than normal saline control group. Lactobacillus treated groups and triple treated group showed significant decrease of H. pylori bacterial density.

CONCLUSION: Both lactobacillus strains have a significant anti-H. pylori activity; L. fermenti displays more efficient antagonistic activity in vivo against H. pylori infection.

Keywords: Helicobacter pylori; Lactobacillus fermenti; Murine gastritis; Lactobacillus acidophilus; Therapy