Mendoza JL, San-Pedro A, Culebras E, Cíes R, Taxonera C, Lana R, Urcelay E, Torre FL, Picazo JJ, Díaz-Rubio M. High prevalence of viable Mycobacterium avium subspecies paratuberculosis in Crohn’s disease. World J Gastroenterol 2010; 16(36): 4558-4563 [PMID: 20857526 DOI: 10.3748/wjg.v16.i36.4558]
Corresponding Author of This Article
Juan L Mendoza, MD, PhD, Department of Gastroenterology, Hospital Clinico San Carlos, Martin Lagos s/n, 28040 Madrid, Spain. jmendozah@meditex.es
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World J Gastroenterol. Sep 28, 2010; 16(36): 4558-4563 Published online Sep 28, 2010. doi: 10.3748/wjg.v16.i36.4558
High prevalence of viable Mycobacterium avium subspecies paratuberculosis in Crohn’s disease
Juan L Mendoza, Amparo San-Pedro, Esther Culebras, Raquel Cíes, Carlos Taxonera, Raquel Lana, Elena Urcelay, Fernando de la Torre, Juan J Picazo, Manuel Díaz-Rubio
Juan L Mendoza, Carlos Taxonera, Manuel Díaz-Rubio, Department of Gastroenterology, Hospital Clinico San Carlos, 28040 Madrid, Spain
Amparo San-Pedro, Esther Culebras, Raquel Cíes, Fernando de la Torre, Juan J Picazo, Department of Clinical Microbiology, Hospital Clinico San Carlos, 28040 Madrid, Spain
Raquel Lana, Department of Internal Medicine, Hospital Clinico San Carlos, 28040 Madrid, Spain
Elena Urcelay, Department of Immunology, Hospital Clinico San Carlos, 28040 Madrid, Spain
Author contributions: San-Pedro A, Culebras E, Cíes R, de la Torre F and Urcelay E performed the majority of experiments; Mendoza JL and Lana R provided the collection of human material for this work; Mendoza JL, Taxonera C, Culebras E, Picazo JJ and Díaz-Rubio M designed the study and wrote the manuscript; Díaz-Rubio M provided financial support for this work.
Supported by A grant from Fundación de Investigación Médica Mutua Madrileña (FMM) (to Díaz-Rubio M)
Correspondence to: Juan L Mendoza, MD, PhD, Department of Gastroenterology, Hospital Clinico San Carlos, Martin Lagos s/n, 28040 Madrid, Spain. jmendozah@meditex.es
Telephone: +34-91-3303693 Fax: +34-91-3303757
Received: April 28, 2010 Revised: June 15, 2010 Accepted: June 22, 2010 Published online: September 28, 2010
Abstract
AIM: To examine the detection rate of viable Mycobacterium avium subspecies paratuberculosis (MAP) in patients with inflammatory bowel disease [Crohn’s disease (CD) and ulcerative colitis (UC)].
METHODS: Thirty patients with CD (15 with at least one NOD2/CARD15 mutation), 29 with UC, and 10 with no inflammatory bowel disease (IBD). were tested for MAP by polymerase chain reaction (specific IS900 fragment) and blood culture.
RESULTS: MAP DNA was detected in all original blood samples and 8-wk blood cultures (CD, UC and non-IBD). Positive MAP DNA status was confirmed by dot blot assays. All 69 cultures were negative by acid-fast Ziehl-Neelsen staining. Viable MAP, in spheroplast form, was isolated from the 18-mo blood cultures of all 30 CD patients, one UC patient, and none of the non-IBD controls. No association was found between positive MAP cultures and use of immunosuppressive drugs or CD-associated single nucleotide polymorphisms.
CONCLUSION: MAP is widely present in our area and MAP DNA can be recovered from the blood of CD, UC and non-IBD patients. However, MAP spheroplasts were only found in CD patients.
