Lopez-Font I, Gea-Sorlí S, de-Madaria E, Gutiérrez LM, Pérez-Mateo M, Closa D. Pancreatic and pulmonary mast cells activation during experimental acute pancreatitis. World J Gastroenterol 2010; 16(27): 3411-3417 [PMID: 20632444 DOI: 10.3748/wjg.v16.i27.3411]
Corresponding Author of This Article
Dr. Daniel Closa, Department of Experimental Pathology, Institute of Biomedical Research of Barcelona-Spanish National Research Council (IIBB-CSIC), Institute of Biomedical Research August Pi Sunyer (IDIBAPS) and Biomedical Research Centre Network on Liver and Digestive Diseases, Barcelona (CIBEREHD), c/ Rosselló 161, 7º, Barcelona 08036, Spain. dclbam@iibb.csic.es
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World J Gastroenterol. Jul 21, 2010; 16(27): 3411-3417 Published online Jul 21, 2010. doi: 10.3748/wjg.v16.i27.3411
Pancreatic and pulmonary mast cells activation during experimental acute pancreatitis
Inmaculada Lopez-Font, Sabrina Gea-Sorlí, Enrique de-Madaria, Luis M Gutiérrez, Miguel Pérez-Mateo, Daniel Closa
Inmaculada Lopez-Font, Enrique de-Madaria, Miguel Pérez-Mateo, Gastroenterology Unit, University General Hospital, Alicante, 03010, Spain
Sabrina Gea-Sorlí, Daniel Closa, Department of Experimental Pathology, Institute of Biomedical Research of Barcelona-Spanish National Research Council (IIBB-CSIC), Institute of Biomedical Research August Pi Sunyer (IDIBAPS) and Biomedical Research Centre Network on Liver and Digestive Diseases, Barcelona (CIBEREHD), 08036, Spain
Luis M Gutiérrez, Neurosciences Institute, Miguel Hernandez University-Spanish National Research Council (UMH-CSIC), Sant Joan d’Alacant, Alicante, 03550, Spain
Miguel Pérez-Mateo, Miguel Hernandez University, School of Medicine, Elche, 03202, Spain
Author contributions: Lopez-Font I and Gea-Sorlí S contributed equally to this work and carried out the majority of experiments; Pérez-Mateo M, de-Madaria E, Gutiérrez LM and Closa D designed the study; de-Madaria E and Closa D wrote the manuscript.
Supported by The Project SAF2006-08449 and a grant from Fundación para la Investigación del Hospital General Universitario de Alicante; Inmaculada Lopez-Font has a Juan de la Cierva contract supported by the Spanish Ministry of Science and Innovation
Correspondence to: Dr. Daniel Closa, Department of Experimental Pathology, Institute of Biomedical Research of Barcelona-Spanish National Research Council (IIBB-CSIC), Institute of Biomedical Research August Pi Sunyer (IDIBAPS) and Biomedical Research Centre Network on Liver and Digestive Diseases, Barcelona (CIBEREHD), c/ Rosselló 161, 7º, Barcelona 08036, Spain. dclbam@iibb.csic.es
Telephone: +34-93-3638307 Fax: +34-93-3638301
Received: January 13, 2010 Revised: March 25, 2010 Accepted: April 1, 2010 Published online: July 21, 2010
Abstract
AIM: To study the activation of pancreatic and pulmonary mast cells and the effect of mast cell inhibition on the activation of peritoneal and alveolar macrophages during acute pancreatitis.
METHODS: Pancreatitis was induced by intraductal infusion of 5% sodium taurodeoxycholate in rats. The mast cell inhibitor cromolyn was administered intraperitoneally (i.p.) 30 min before pancreatitis induction. The pancreatic and pulmonary tissue damage was evaluated histologically and mast cells and their state of activation were evaluated. Peritoneal and alveolar macrophages were obtained and the expression of tumor necrosis factor α was determined. Myeloperoxidase activity was measured to evaluate the effect of mast cell inhibition on the progression of the inflammatory process. Finally, the effect of plasma on cultured mast cells or macrophages was evaluated in vitro.
RESULTS: The mast cell stabilizer significantly reduced inflammation in the pancreas and lung and the activation of alveolar macrophages but had no effect on peritoneal macrophages. Mast cell degranulation was observed in the pancreas during pancreatitis but no changes were observed in the lung. Plasma from rats with pancreatitis could activate alveolar macrophages but did not induce degranulation of mast cells in vitro.
CONCLUSION: Pancreatic mast cells play an important role in triggering the local and systemic inflammatory response in the early stages of acute pancreatitis. In contrast, lung mast cells are not directly involved in the inflammatory response related to pancreatic damage.
