Original Article
Copyright ©2010 Baishideng. All rights reserved.
World J Gastroenterol. May 21, 2010; 16(19): 2355-2361
Published online May 21, 2010. doi: 10.3748/wjg.v16.i19.2355
Butyrate stimulates IL-32α expression in human intestinal epithelial cell lines
Ayako Kobori, Shigeki Bamba, Hirotsugu Imaeda, Hiromitsu Ban, Tomoyuki Tsujikawa, Yasuharu Saito, Yoshihide Fujiyama, Akira Andoh
Ayako Kobori, Shigeki Bamba, Hirotsugu Imaeda, Hiromitsu Ban, Tomoyuki Tsujikawa, Yasuharu Saito, Yoshihide Fujiyama, Division of Gastroenterology, Department of Internal Medicine, Shiga University of Medical Science, Seta Tukinowa, Otsu 520-2192, Japan
Akira Andoh, Division of Mucosal Immunology, Graduate School of Medicine, Shiga University of Medical Science, Seta Tukinowa, Otsu 520-2192, Japan
Author contributions: Andoh A and Fujiyama Y designed the research; Kobori A, Bamba S, Imaeda H, Ban H, Tsujikawa T and Saito Y performed the research; Andoh A wrote the paper.
Correspondence to: Shigeki Bamba, MD, PhD, Division of Gastroenterology, Department of Internal Medicine, Shiga University of Medical Science, Seta Tukinowa, Otsu, Shiga, 520-2192, Japan. sb@belle.shiga-med.ac.jp
Telephone: +81-77-5482217 Fax: +81-77-5482219
Received: January 10, 2010
Revised: February 23, 2010
Accepted: March 2, 2010
Published online: May 21, 2010
Abstract

AIM: To investigate the effects of butyrate on interleukin (IL)-32α expression in epithelial cell lines.

METHODS: The human intestinal epithelial cell lines HT-29, SW480, and T84 were used. Intracellular IL-32α was determined by Western blotting analyses. IL-32α mRNA expression was analyzed by real-time polymerase chain reaction.

RESULTS: Acetate and propionate had no effects on IL-32α mRNA expression. Butyrate significantly enhanced IL-32α expression in all cell lines. Butyrate also up-regulated IL-1β-induced IL-32α mRNA expression. Butyrate did not modulate the activation of phosphatidylinositol 3-kinase (PI3K), a mediator of IL-32α expression. Like butyrate, trichostatin A, a histone deacetylase inhibitor, also enhanced IL-1β-induced IL-32α mRNA expression.

CONCLUSION: Butyrate stimulated IL-32α expression in epithelial cell lines. An epigenetic mechanism, such as histone hyperacetylation, might be involved in the action of butyrate on IL-32α expression.

Keywords: Cytokine; Histone acetylation; Inflammatory bowel disease