Zeng Z, Huang HF, Chen MQ, Song F, Zhang YJ. Heme oxygenase-1 protects donor livers from ischemia/reperfusion injury: The role of Kupffer cells. World J Gastroenterol 2010; 16(10): 1285-1292 [PMID: 20222175 DOI: 10.3748/wjg.v16.i10.1285]
Corresponding Author of This Article
Zhong Zeng, MD, Organ Transplantation Center, the First Affiliated Hospital of Kunming Medical College, 295 Xichang Road, Kunming 650032, Yunnan Province, China. zzong@medmail.com.cn
Article-Type of This Article
Brief Article
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Zhong Zeng, Han-Fei Huang, Ming-Qing Chen, Fei Song, Organ Transplantation Center, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China
Yu-Jun Zhang, Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
Author contributions: Zeng Z and Huang HF contributed equally to this work; Zeng Z, Huang HF and Chen MQ designed the research; Huang HF, Song F and Zhang YJ performed the research; Huang HF and Chen MQ contributed analytic tools; Song F analyzed the data; Zeng Z and Huang HF wrote the paper.
Supported by The Natural Science Foundation of Yunnan Province, China, No. 2007C137M and the Joint Funds of Natural Science Foundation of Yunnan Province, China, No. 2007C0009R
Correspondence to: Zhong Zeng, MD, Organ Transplantation Center, the First Affiliated Hospital of Kunming Medical College, 295 Xichang Road, Kunming 650032, Yunnan Province, China. zzong@medmail.com.cn
Telephone: +86-871-5359202 Fax: +86-871-5359202
Received: November 21, 2009 Revised: January 3, 2010 Accepted: January 10, 2010 Published online: March 14, 2010
Abstract
AIM: To examine whether heme oxygenase (HO)-1 overexpression would exert direct or indirect effects on Kupffer cells activation, which lead to aggravation of reperfusion injury.
METHODS: Donors were pretreated with cobalt protoporphyrin (CoPP) or zinc protoporphyrin (ZnPP), HO-1 inducer and antagonist, respectively. Livers were stored at 4°C for 24 h before transplantation. Kupffer cells were isolated and cultured for 6 h after liver reperfusion.
RESULTS: Postoperatively, serum transaminases were significantly lower and associated with less liver injury when donors were pretreated with CoPP, as compared with the ZnPP group. Production of the cytokines tumor necrosis factor-α and interleukin-6 generated by Kupffer cells decreased in the CoPP group. The CD14 expression levels (RT-PCR/Western blots) of Kupffer cells from CoPP-pretreated liver grafts reduced.
CONCLUSION: The study suggests that the potential utility of HO-1 overexpression in preventing ischemia/reperfusion injury results from inhibition of Kupffer cells activation.
