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World J Gastroenterol. Aug 21, 2008; 14(31): 4943-4948
Published online Aug 21, 2008. doi: 10.3748/wjg.14.4943
Expression transformation of claudin-1 in the process of gastric adenocarcinoma invasion
Yong-Lian Wu, Sheng Zhang, Guo-Rong Wang, Yu-Peng Chen
Yong-Lian Wu, The Medical College of Putian University, Putian 351100, Fujian Province, China
Sheng Zhang, Yu-Peng Chen, Department of Pathology, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, Fujian Province, China
Guo-Rong Wang, Hanjiang Hospital, Putian 351100, Fujian Province, China
Author contributions: Wu YL and Zhang S contributed equally to this work; Wu YL and Zhang S designed the research; Wu YL, Zhang S, Wang GR and Chen YP performed the research; Chen YP contributed to new reagents; Wu YL and Zhang S analyzed the data and wrote the paper.
Supported by The Science Foundation of Putian City, Fujian Province, China, No. 2006D01
Correspondence to: Dr. Sheng Zhang, Department of Patho-logy, the First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, Fujian Province, China. zhgshg@126.com
Telephone: +86-591-87982733 Fax: +86-591-87982028
Received: May 26, 2008
Revised: July 14, 2008
Accepted: July 21, 2008
Published online: August 21, 2008
Abstract

AIM: To investigate the relation of expression transformation of claudin-1 with invasiveness and metastasis of gastric carcinoma.

METHODS: By using immunohistochemistry, expression of claudin-1 in mucosa and invasive front of 136 gastric adenocarcinoma cases and proliferative index (Ki-67) were detected and analyzed.

RESULTS: In mucosa, the claudin-1 over-expression rate of mucinous adenocarcinomas (including signet-ring cell carcinomas) was the highest. It was negatively related with the differentiation but positively related with the invasiveness and metastasis of gastric cancer. In invasive front, the claudin-1 over-expression rate was positively related with the differentiation, invasiveness and metastasis of gastric carcinoma. The expression transformation of claudin-1 was found in gastric carcinoma. The expression of claudin-1 in invasive front was transformed in 28/136 gastric carcinoma cases. The transformation rate in highly differentiated tubular adenocarcinomas was the highest (51.5%, 17/33). The deeper was the invasiveness, the higher was the transformation rate. The claudin-1 expression transformation rate in serosa and omenta was significantly higher (92.9%) than in tunica muscularis of invasive gastric cancer cases, as well as in patients with lymph node metastasis than in those without lymph node metastasis.

CONCLUSION: Up-regulation of claudin-1 expression and its transformation in invasive and metastatic gastric carcinoma suggest that claudin-1 participates in the transformation of biological behaviors in neoplasms. Further study is needed to elucidate the precise mechanism and the relation of claudin-1 expression with the neoplasm progress.

Keywords: Gastric carcinoma; Claudin-1; Expression transformation; Invasiveness; Metastasis; Immunohistochemistry