Mao ZQ, Huang Y, Sun M, Ruan Q, Qi Y, He R, Huang YJ, Ma YP, Ji YH, Sun ZR, Gao H. Genetic polymorphism of UL144 open reading frame of human cytomegalovirus DNA detected in colon samples from infants with Hirschsprung’s disease. World J Gastroenterol 2007; 13(32): 4350-4354 [PMID: 17708610 DOI: 10.3748/wjg.v13.i32.4350]
Corresponding Author of This Article
Dr. Zhi-Qin Mao, Department of Pediatrics, Second Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning Province, China. m_zq2004@163.com
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Rapid Communication
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World J Gastroenterol. Aug 28, 2007; 13(32): 4350-4354 Published online Aug 28, 2007. doi: 10.3748/wjg.v13.i32.4350
Genetic polymorphism of UL144 open reading frame of human cytomegalovirus DNA detected in colon samples from infants with Hirschsprung’s disease
Zhi-Qin Mao, Ying Huang, Mei Sun, Qiang Ruan, Ying Qi, Rong He, Yu-Jing Huang, Yan-Ping Ma, Yao-Hua Ji, Zheng-Rong Sun, Hong Gao
Zhi-Qin Mao, Mei Sun, Department of Pediatrics, Second Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning Province, China
Ying Huang, Hong Gao, Department of Pediatric Surgery, Second Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning Province, China
Qiang Ruan, Rong He, Ying Qi, Yu-Jing Huang, Yan-Ping Ma, Yao-Hua Ji, Zheng-Rong Sun, Virus Laboratory, Second Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning Province, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No.30170986
Correspondence to: Dr. Zhi-Qin Mao, Department of Pediatrics, Second Affiliated Hospital, China Medical University, Shenyang 110004, Liaoning Province, China. m_zq2004@163.com
Telephone: +86-24-83955570
Received: April 3, 2007 Revised: April 23, 2007 Accepted: April 26, 2007 Published online: August 28, 2007
Abstract
AIM: To explore the genetic diversities of UL144 open reading frame (ORF) of cytomegalovirus DNA detected in colon tissue from infants with Hirschsprung’s disease (HD) by sequencing UL144 DNA in 23 aganglionic colon tissue and 4 urine samples from 25 HD infants.
METHODS: Nest PCR was performed for amplification of the UL144 gene. The UL144 gene was analyzed with softwares, such as DNAclub, BioEdit, PROSITE database, and DNAstar.
RESULTS: The strains from HD patients were distributed among three genotypes of UL144: group 1A (64%), group 2 (24%), and group 3 (12%). The UL144 genotypes between strains from HD and control group were compared by chi square test (χ2 = 1.870, P = 0.393). Strains from the colon were sporadically distributed in UL144 genotypes.
CONCLUSION: There are genetic diversities of UL144 ORF in colon tissue of infants with HD. However, cytomegalovirus UL144 genotypes are not associated with clinical manifestations of HD.
