Basic Research
Copyright ©2006 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Apr 7, 2006; 12(13): 2024-2030
Published online Apr 7, 2006. doi: 10.3748/wjg.v12.i13.2024
Rosuvastatin reduces rat intestinal ischemia-reperfusion injury associated with the preservation of endothelial nitric oxide synthase protein
Yuji Naito, Kazuhiro Katada, Tomohisa Takagi, Hisato Tsuboi, Masaaki Kuroda, Osamu Handa, Satoshi Kokura, Norimasa Yoshida, Hiroshi Ichikawa, Toshikazu Yoshikawa
Yuji Naito, Toshikazu Yoshikawa, Department of Medical Proteomics, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan
Kazuhiro Katada, Hisato Tsuboi, Masaaki Kuroda, Toshikazu Yoshikawa, Inflammation and Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan
Tomohisa Takagi, Osamu Handa, Satoshi Kokura, Toshikazu Yoshikawa, Department of Biomedical Safety Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan
Norimasa Yoshida, Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan
Hiroshi Ichikawa, Department of Food Sciences and Nutritional Health, The Faculty of Human Environment, Kyoto Prefectural University, Kyoto 606-8522, Japan
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Yuji Naito, Department of Medical Proteomics, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan. ynaito@koto.kpu-m.ac.jp
Telephone: +81-75-2515505 Fax: +81-75-2523721
Received: August 25, 2005
Revised: November 11, 2005
Accepted: November 18, 2005
Published online: April 7, 2006
Abstract

AIM: To investigate the protective effect of rosuvastatin on ischemia-reperfusion (I-R)-induced small intestinal injury and inflammation in rats, and to determine the effect of this agent on the expression of endothelial nitric oxide synthase (eNOS) protein.

METHODS: Intestinal damage was induced in male Sprague-Dawley rats by clamping both the superior mesenteric artery and the celiac trunk for 30 min, followed by reperfusion for 60 min. Rosuvastatin dissolved in physiological saline was administered intraperitoneally 60 min before ischemia. The severity of the intestinal mucosal injury and inflammation were evaluated by several biochemical markers, as well as by histological findings. The protein levels of eNOS were determined by Western blot.

RESULTS: The levels of both intraluminal hemoglobin and protein, as indices of mucosal damage, were significantly increased in the I-R group compared with those in the sham-operated group. These increases, however, were significantly inhibited by treatment with rosuvastatin in a dose-dependent manner. The protective effects of rosuvastatin were also confirmed by histological findings. Exposure of the small intestine to I-R resulted in mucosal inflammation characterized by significant increases in thiobarbituric acid-reactive substances, tissue-associated myeloperoxidase activity, and the mucosal contents of rat cytokine-induced neutrophil chemoattractant-1 (CINC-1) and tumor necrosis factor-α (TNF-α). These increases in inflammatory parameters after I-R were significantly inhibited by pretreatment with rosuvastatin at a dose of 10 mg/kg. Furthermore, mRNA expression of CINC-1 and TNF-α was increased after I-R, and this increase was also inhibited by rosuvastatin. The mucosal protein levels of eNOS decreased during I-R, but were preserved in rats treated with rosuvastatin.

CONCLUSION: Rosuvastatin inhibits rat intestinal injury and inflammation induced by I-R, and its protection is associated with the preservation of eNOS protein.

Keywords: Rosuvastatin; Rat; Intestine; Ischemia-reperfusion injury; Endothelium; Nitric oxide synthase