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World J Gastroenterol. Nov 7, 2005; 11(41): 6549-6553
Published online Nov 7, 2005. doi: 10.3748/wjg.v11.i41.6549
No relationship between IL-1B gene polymorphism and gastric acid secretion in younger healthy volunteers
Sheng Hu, Qi-Bing Song, Ping-Fang Yao, Qing-Long Hu, Ping-Jin Hu, Zhi-Rong Zeng, Rui-Ping Pang
Sheng Hu, Qi-Bing Song, Ping-Fang Yao, Qing-Long Hu, Zhi-Rong Zeng, Rui-Ping Pang, Institute of Cancer, the Cancer Hospital of Hubei Province, Wuhan 430079, Hubei Province, China
Qing-Long Hu, Department of Pathology, Creighton University Medical Center, 30th Street, Omaha NE 68131, United States
Ping-Jin Hu, Zhi-Rong Zeng, Rui-Ping Pang, Department of Gastroenterology, the First Affiliated Hospital of Sun Yat-Sen University of Medical Science, Guangzhou 510080, Guangdong Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Hu Sheng, Institute of Cancer, the Cancer Hospital of Hubei Province, Wuhan 430079, Hubei Province, China. ehusmn@yahoo.com.cn
Telephone: +86-27-87670057
Received: January 12, 2005
Revised: April 15, 2005
Accepted: April 18, 2005
Published online: November 7, 2005
Abstract

AIM: To investigate the influence of IL-1B-511 gene polymorphism on IL-1B mRNA expression and gastric acid output in individual with or without Helicobacter pylori (H pylori) infection.

METHODS: IL-1B mRNA expression and gastric acid secretion in 117 health volunteers were assayed using semi-quantitative RT-PCR and gastric juice assay, respectively. Pepsinogen (PG) I and II of 255 subjects (including 117 health volunteers) were also examined.

RESULTS: T/T genotype individuals with H pylori infection had a more decreased PG I/II ratio. In gastric antrum mucosa, the individuals with H pylori infection had higher IL-1B expression than those without H pylori infection, but there was no obvious difference among each genotype. In gastric corpus, the individuals with H pylori infection had a significantly higher IL-1B expression than those without H pylori infection. IL-1B-511T/T genotype was markedly higher as compared with the other two genotypes. Both maximal acid output and basic acid output were similar among each genotype in IL-1B-511 gene locus, regardless of H pylori infection.

CONCLUSION: IL-1B-511 T allele does not decrease gastric acid output, although it has a stimulated influence on IL-1B expression. Consequently, the pathway, through which IL-1B plays a central role in gastric cancer development, might not depend on low acid, but on the other regulation mechanisms.

Keywords: IL-1; Polymorphism; Stomach neoplasm; Gastric acid; Helicobacter pylori (H pylori)