Brief Reports
Copyright ©The Author(s) 2005. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jul 14, 2005; 11(26): 4098-4101
Published online Jul 14, 2005. doi: 10.3748/wjg.v11.i26.4098
Comparative study on radiosensitivity of various tumor cells and human normal liver cells
Jian-She Yang, Wen-Jian Li, Guang-Ming Zhou, Xiao-Dong Jin, Jing-Guang Xia, Ju-Fang Wang, Zhuan-Zi Wang, Chuan-Ling Guo, Qing-Xiang Gao
Jian-She Yang, Wen-Jian Li, Guang-Ming Zhou, Xiao-Dong Jin, Jing-Guang Xia, Ju-Fang Wang, Zhuan-Zi Wang, Chuan-Ling Guo, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, Gansu Province, China
Qing-Xiang Gao, Life Science School of Lanzhou University, Lanzhou 730000, Gansu Province, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 10335050; Ministry of Science and Technology of the People’s Republic of China, No. 2003CCB00200
Correspondence to: Dr. Jian-She Yang, Radiobiology Laboratory, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, Gansu Province, China. tuyangjs@impcas.ac.cnit
Telephone: +86-931-4969338 Fax: +86-931-4969201
Received: November 3, 2004
Revised: November 14, 2004
Accepted: November 19, 2004
Published online: July 14, 2005
Abstract

AIM: To investigate the radiation response of various human tumor cells and normal liver cells.

METHODS: Cell lines of human hepatoma cells (SMMC-7721), liver cells (L02), melanoma cells (A375) and cervical tumor (HeLa) were irradiated with 60Co γ-rays. Cell survive was documented by a colony assay. Chromatid breaks were measured by counting the number of chromatid breaks and isochromatid breaks immediately after prematurely chromosome condensed by Calyculin-A.

RESULTS: Linear quadratic survival curve was observed in all of four cell lines, and dose-dependent increase in radiation-induced chromatid and isochromatid breaks were observed in GB2B phase. Among these four cell lines, A375 was most sensitive to radiation, while, L02 had the lowest radiosensitivity. For normal liver cells, chromatid breaks were easy to be repaired, isochromatid breaks were difficult to be repaired.

CONCLUSION: The results suggest that the γ-rays induced chromatid breaks can be possibly used as a good predictor of radiosensitivity, also, unrejoined isochromatid breaks probably tightly related with cell cancerization.

Keywords: Radiosensitivity, Tumor cells, Normal, Liver cells