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World J Gastroenterol. Jan 14, 2005; 11(2): 293-295
Published online Jan 14, 2005. doi: 10.3748/wjg.v11.i2.293
Mechanism of benign biliary stricture: A morphological and immunohistochemical study
Zhi-Min Geng, Ying-Min Yao, Qing-Guang Liu, Xin-Jie Niu, Xiao-Gong Liu
Zhi-Min Geng, Ying-Min Yao, Qing-Guang Liu, Xin-Jie Niu, Xiao-Gong Liu, Department of Hepatobiliary Surgery, First Hospital of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Zhi-Min Geng, Department of Hepatobiliary Surgery, First Hospital of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China. zhimin@pub.xaonline.com
Telephone: +86-29-85324009 Fax: +86-29-85324009
Received: March 2, 2003
Revised: March 5, 2003
Accepted: April 3, 2003
Published online: January 14, 2005
Abstract

AIM: To explore the mechanism of benign biliary stricture.

METHODS: A model of trauma of bile duct was established in 28 dogs. The anastomosed tissues were resected and examined by light and electron microscopes on day 3, in wk 1, 3 and mo 3, 6 after operation. CD68, TGF-β1 and α-SMA were examined by immunohistochemical staining, respectively.

RESULTS: The mucosal epithelium of the bile duct was slowly recovered, chronic inflammation lasted for a long time, fibroblasts proliferated actively, extracellular matrix was over-deposited. Myofibroblasts functioned actively and lasted through the whole process. The expression of macrophages in lamina propria under mucosa, TGF-β1 in granulation tissue, fibroblasts and endothelial cells of blood vessels, α-SMA in myofiroblasts were rather strong from the 1st wk to the 6th mo after operation.

CONCLUSION: The type of healing occurring in bile duct belongs to overhealing. Myofibroblasts are the main cause for scar contracture and stricture of bile duct. High expressions of CD68, TGF-β1 and α-SMA are closely related to the active proliferation of fibroblasts, extracellular matrix over-deposition and scar contracture of bile duct.

Keywords: Benign biliary stricture; Immunohistochemistry