Liver Cancer
Copyright ©2005 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Jan 14, 2005; 11(2): 212-215
Published online Jan 14, 2005. doi: 10.3748/wjg.v11.i2.212
Adhesion of different cell cycle human hepatoma cells to endothelial cells and roles of integrin β1
Guan-Bin Song, Jian Qin, Qing Luo, Xiao-Dong Shen, Run-Bin Yan, Shao-Xi Cai
Guan-Bin Song, Jian Qin, Qing Luo, Xiao-Dong Shen, Run-Bin Yan, Shao-Xi Cai, Key Laboratory for Biomechanics and Tissue Engineering of the State Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No.19972077 and No.10372121
Correspondence to: Dr. Guan-Bin Song, Key Laboratory for Biomechanics and Tissue Engineering of the State Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China. song9973@tom.com
Telephone: +86-23-65102507 Fax: +86-23-65111633
Received: February 2, 2004
Revised: February 5, 2004
Accepted: April 5, 2004
Published online: January 14, 2005
Abstract

AIM: To investigate the adhesive mechanical properties of different cell cycle human hepatoma cells (SMMC-7721) to human umbilical vein endothelial cells (ECV-304), expression of adhesive molecule integrinβ1 in SMMC-7721 cells and its contribution to this adhesive course.

METHODS: Adhesive force of SMMC-7721 cells to endothelial cells was measured using micropipette aspiration technique. Synchronous G1 and S phase SMMC-7721 cells were achieved by thymine-2-deoxyriboside and colchicines sequential blockage method and double thymine-2-deoxyriboside blockage method, respectively. Synchronous rates of SMMC-7721 cells and expression of integrinβ1 in SMMC-7721 cells were detected by flow cytometer.

RESULTS: The percentage of cell cycle phases of general SMMC-7721 cells was 11.01% in G2/M phases, 53.51% in G0/G1 phase, and 35.48% in S phase. The synchronous rates of G1 and S phase SMMC-7721 cells amounted to 74.09% and 98.29%, respectively. The adhesive force of SMMC-7721 cells to endothelial cells changed with the variations of adhesive time and presented behavior characteristics of adhesion and de-adhesion. S phase SMMC-7721 cells had higher adhesive forces than G1 phase cells [(307.65±92.10)×10-10N vs (195.42±60.72)×10-10N, P<0.01]. The expressive fluorescent intensity of integrinβ1 in G1 phase SMMC-7721 cells was depressed more significantly than the values of S phase and general SMMC-7721cells. The contribution of adhesive integrinβ1 was about 53% in this adhesive course.

CONCLUSION: SMMC-7721 cells can be synchronized preferably in G1 and S phases with thymine-2-deoxyriboside and colchicines. The adhesive molecule integrinβ1 expresses a high level in SMMC-7721 cells and shows differences in various cell cycles, suggesting integrin β1 plays an important role in adhesion to endothelial cells. The change of adhesive forces in different cell cycle SMMC-7721 cells indicates that S phase cells play predominant roles possibly while they interact with endothelial cells.

Keywords: Hepatoma; Cell cycle; Integrin β1; Endothelial cells; Cell adhesion